We narrowed to 9,752 results for: try
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Plasmid#108859PurposeFnCpf1 Gateway entry plasmidDepositorInsertFnCpf1
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationCpf1 is rice codon optimizedAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-FLAG-CYLD-C601A
Plasmid#60029PurposeCYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tagDepositorInsertCylindromatosis (CYLD Human)
TagsFLAGExpressionMammalianMutationCysteine 601 changed to AlaninePromoterTREAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1
Plasmid#121831PurposepMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/LSD1 (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
374 pME-ets1a
Plasmid#49000Purposefull length coding sequence for zebrafish ets1a gene in gateway middle entry vectorDepositorInsertv-ets erythroblastosis virus E26 oncogene homolog 1a (ets1 Zebrafish)
UseGateway middle entryAvailable SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
SLK_HUMAN_D0
Plasmid#79733PurposeThis plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265G2
Plasmid#164723PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-ziSpyMac(D10A)-UGI)
UseCRISPR; Gateway compatible a3a/y130f-ispymaccas9-…ExpressionPlantAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
(p)odr-3::GFP::EGL-4
Plasmid#100897PurposeGFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.DepositorInsert56-2741: odr-3 promoter
ExpressionWormPromoterodr-3Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKK-HA-TEV
Plasmid#105769PurposeExpression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsHA-TEVExpressionMammalianAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ165
Plasmid#109327PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterPromoter EC1.2 enhancer fused to EC1.1 promoterAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-HA
Plasmid#105785PurposeExpression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-HAExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP2.GFP
Plasmid#109140PurposeGFP-tagged CYFIP2 expression constructDepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
139H2_LC
Plasmid#206202PurposeFor recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.DepositorAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CCL5.Pro
Plasmid#79492PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoterDepositorInsertCCL5 Promoter (CCL5 Human, Synthetic)
UseMultisite gateway entry clonePromoterCCL5 PromoterAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L
Plasmid#121833PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/Dnmt3a-3L (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT JunD-HA
Plasmid#58498PurposeGateway entry vector for an inducible HA-tagged mouse JunDDepositorAvailable SinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m
Plasmid#161522PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editingDepositorInsertTadA(wt)-TadA(7.10)-zSpCas9(D10A)
UseCRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…ExpressionPlantAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR3c GW CHD5
Plasmid#68869PurposeGW entry vector for CHD5DepositorAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
Plasmid#194755PurposeLentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchorDepositorInsertPrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS
UseLentiviralTagsHAPromoterCMVAvailable SinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-DrCav1
Plasmid#194290PurposeMultisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRTDepositorInsertcaveolin1 (cav1.S Frog)
UseMultisite gateway entry vectorAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPRR2A-pFastBac I
Plasmid#182460PurposeExpression His-tagged SPRR2A in insect cellsDepositorInsertsmall proline rich protein 2A (SPRR2A Human)
Tags6xHisExpressionInsectPromoterPolyhedrinAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only