We narrowed to 5,888 results for: ARL

1. pJW1821

   Plasmid

   #163092

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mScarlet^SEC (Lox511I)^::3xMyc
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pECE M2-SH3PXD2A 8 serines mutated to alanines

   Plasmid

   #69816

   Purpose

   Expresses M2-SH3PXD2A in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTet-O-ATOH7-T2A-PuroR

   Plasmid

   #162343

   Purpose

   Lentiviral expression of ATOH7 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   ATOH7 (ATOH7 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pECE M2-SH3PXD2A 6 serines mutated to alanines

   Plasmid

   #69815

   Purpose

   Expresses M2-SH3PXD2A in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS252

   Plasmid

   #33207

   Depositor

   David Bartel

   Article

   Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

   Insert

   MAP4K4 3'UTR (MAP4K4 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   miR-CGCG sites: aaCgCga, aaCgCgaa

   Promoter

   SV40 early enhancer/promoter

   Available since

   Jan. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

   Plasmid

   #220597

   Purpose

   Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   mVenus-Q69M (ME)
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

   Plasmid

   #173891

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDF11-SFPQ(214-598)_quadruple_mutant

   Plasmid

   #135441

   Purpose

   Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   splicing factor proline and glutamine rich (SFPQ Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   L535A, L539A, L546A, M549A

   Promoter

   T7

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-HDAC4*

   Plasmid

   #71402

   Purpose

   CAG promoter, mammalian expression

   Depositor

   Bo Chen

   Article

   Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

   Insert

   HDAC4* (HDAC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   After aa126, insertion leads to reading frame shi…

   Promoter

   CAG

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

10. 1GFP/RNase H1 D210N

    Plasmid

    #174448

    Purpose

    Express GFP-tagged RNase H1 D210N in E. coli.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    RNase H1 (RNASEH1 Human)
    

    Use

    Tags

    His-tag, GFP

    Expression

    Bacterial

    Mutation

    D210N

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-PalmGRET

    Plasmid

    #158221

    Purpose

    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

    Depositor

    Charles P. Lai

    Article

    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

    Insert

    PalmGFP-Nluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGolt-mCherry / pGolt3-mCherry

    Plasmid

    #73297

    Purpose

    Visualization of Dendritic Golgi Satellite

    Depositor

    Michael Kreutz

    Article

    Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

    Inserts

    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    

    Use

    Tags

    ER export and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-nEF-Coff/Fon DREADD Gi-mCherry

    Plasmid

    #177669

    Purpose

    Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

    Depositor

    Karl Deisseroth

    Article

    Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

    Insert

    DREADD Gi-mCherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting ; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTet-O-ASCL1-T2A-PuroR

    Plasmid

    #162345

    Purpose

    Lentiviral expression of ASCL1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    ASCL1 (ASCL1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-Ef1a-Con/Fon-mCherry

    Plasmid

    #137132

    Purpose

    Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-EF1a-mitoGFP-DIO

    Plasmid

    #174112

    Purpose

    Cre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoter

    Depositor

    Maarten Kole

    Article

    Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

    Insert

    mitoGFP (COX8A Human)
    

    Use

    AAV

    Tags

    Cox8 targeting sequence and GFP

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NST1_pOpOn2.1

    Plasmid

    #228065

    Purpose

    Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

    Depositor

    Charles Anderson

    Article

    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

    Insert

    NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
    

    Use

    Dexamethasone inducible vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. t1-435

    Plasmid

    #166578

    Purpose

    For expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

    Insert

    Tln1 Head (1-435) (TLN1 Human)
    

    Use

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 1-435 only

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EF1a-DIO-PSD95-EGFP-WPRE

    Plasmid

    #133785

    Purpose

    Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell

    Depositor

    Michael Stryker

    Article

    Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

    Insert

    PSD95-EGFP (Dlg4 Rat)
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-nEF Con/Fon hChR2(H134R)-EYFP

    Plasmid

    #55644

    Purpose

    Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    nEF

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-Duet-1-NONO(53-312)

    Plasmid

    #135442

    Purpose

    Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    Non-POU domain-containing octamer-binding protein (NONO Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-hnEF Coff/Fon hChR2(H134R)-EYFP

    Plasmid

    #55649

    Purpose

    Cre-off/Flp-on ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre off/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Short Ef1

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOTTC337 - pAAV EF1a DIO mCherry

    Plasmid

    #47636

    Purpose

    An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTet-O-ATOH1-T2A-PuroR

    Plasmid

    #162342

    Purpose

    Lentiviral expression of ATOH1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    ATOH1 (ATOH1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

    Plasmid

    #55648

    Purpose

    Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre off/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-Ef1a-Con/Foff 2.0-EYFP

    Plasmid

    #137162

    Purpose

    Intersectional viral expression of EYFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

    Plasmid

    #55646

    Purpose

    Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp off chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-nEF-Con/Fon/Von eYFP

    Plasmid

    #137164

    Purpose

    Intersectional viral expression of EYFP in cells expressing Cre AND Flp AND Vcre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV Retrograde and AAV8

    Insert

    3x-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOTTC364 - pAAV CaMKIIa iRFP

    Plasmid

    #47903

    Purpose

    An AAV vector that expresses iRFP under the CaM Kinase IIa promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Infrared fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-nEF-Con/Fon-iC++-EYFP

    Plasmid

    #137155

    Purpose

    Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-iC++-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge