We narrowed to 20,235 results for: ATO

1. pWZL-hygro-FAKR177/178A

   Plasmid

   #216543

   Purpose

   This retroviral plasmid expresses a mutated cDNA (dominant negative: R177/178A) of human PTK2

   Depositor

   Georgia Konstantinidou

   Article

   Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

   Insert

   PTK2 (R177/178A) (PTK2 Human)
   

   Use

   Retroviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   FAK dominant negative mutation R177/178A

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWR63(YUM1)

   Plasmid

   #203336

   Purpose

   episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   CCW12 promoter (S. stipitis)
   CaCas9
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   THD3 promoter (S. stipitis)
   tRNA-sgRNA(SapI)-HDV
   ADH1 terminator (S. stipitis)
   YUM1-targeting sequence
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWR63

   Plasmid

   #203331

   Purpose

   episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   CCW12 promoter (S. stipitis)
   CaCas9
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   THD3 promoter (S. stipitis)
   tRNA-sgRNA(SapI)-HDV
   ADH1 terminator (S. stipitis)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223-TAF5-FL_WobbleMut

   Plasmid

   #209053

   Purpose

   Gateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNA

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 full length
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-TAF5-∆ex8_WobbleMut

   Plasmid

   #209054

   Purpose

   Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 deltaexon-8
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2702-AGER-gRNA1

   Plasmid

   #216471

   Purpose

   to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   sgRNA targeting human AGER locus
   

   Use

   CRISPR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2703-AGER-gRNA2

   Plasmid

   #216472

   Purpose

   to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   sgRNA targeting human AGER locus
   

   Use

   CRISPR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2704-AGER-gRNA3

   Plasmid

   #216473

   Purpose

   to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   sgRNA targeting human AGER locus
   

   Use

   CRISPR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2705-AGER-eGFP

   Plasmid

   #216474

   Purpose

   donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   eGFP
   

   Use

   CRISPR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

    Plasmid

    #189636

    Purpose

    Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mitoTALECD for_atp1_1333NC

    Plasmid

    #186202

    Purpose

    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

    Inserts

    mitochondrial targeted TALE left hand with 1333N of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333C of Cytidine Deaminase (mitoTALECD)
    

    Use

    Synthetic Biology; Ti plasmid for plant transform…

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mitoTALECD for_atp1_1333CN

    Plasmid

    #186201

    Purpose

    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

    Inserts

    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333B of Cytidine Deaminase (mitoTALECD)
    

    Use

    Synthetic Biology; Ti plasmid for plant transform…

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. HT114_Fsyn_FAS(Cre off)_QuasAr6b_Citrine

    Plasmid

    #178825

    Purpose

    Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    QuasAr6b_citrine
    

    Use

    Cre/Lox and Lentiviral

    Tags

    citrine

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLCHKO_PDPR_exon_deletion_2

    Plasmid

    #155066

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCHKO_SFRS7_exon_deletion_1

    Plasmid

    #155069

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLCHKO_SFRS7_exon_deletion_2

    Plasmid

    #155070

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLCHKO_MDM4_exon_deletion_1

    Plasmid

    #155073

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLCHKO_MDM4_exon_deletion_2

    Plasmid

    #155074

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLCHKO_MDM4_exon_deletion_4

    Plasmid

    #155076

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLCHKO_Ptbp_ex8_1

    Plasmid

    #155081

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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