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We narrowed to 23,601 results for: sequence

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Showing: 3661 - 3680 of 23601 results

  1. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126759
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126759-plasmid-map-sequence-id-412375

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  3. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126754
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126754-plasmid-map-sequence-id-410972

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  4. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

    Plasmid
    #126755
    Purpose
    Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126755-plasmid-map-sequence-id-410971

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  5. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126756
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126756-plasmid-map-sequence-id-410579

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  6. pET24a-VHH-tev-2xTS

    Plasmid
    #117750
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37
    Insert
    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    117750-plasmid-map-sequence-id-228351

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  8. HeFm1SpCas9

    Plasmid
    #92110
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92110-plasmid-map-sequence-id-177882

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  9. SMR3B-bio-His

    Plasmid
    #52183
    Purpose
    Expresses full-length Submaxillary gland androgen-regulated protein 3B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SMR3B (SMR3B Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52183-plasmid-map-sequence-id-80946

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  10. CD84-bio-His

    Plasmid
    #51649
    Purpose
    Expresses full-length SLAM family member 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD84 (CD84 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51649-plasmid-map-sequence-id-78973

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  12. pLenti6.3/EV

    Plasmid
    #120848
    Purpose
    Control vector, 20nt random sequence between gateway recombination sites
    Depositor
    Jan Rehwinkel
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    120848-plasmid-map-sequence-id-234653

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  13. pAAV-CAG-Flex-Rev-3xGFP

    Plasmid
    #122500
    Purpose
    Cre-dependent GFP expression.
    Depositor
    Kimberly Ritola
    Insert
    3x GFP
    Use
    AAV
    Mutation
    each GFP has unique nt sequence
    Available Since
    Jan. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    122500-plasmid-map-sequence-id-237802

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  14. pOpen-HindIIIR

    Plasmid
    #165508
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    HindIII
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165508-plasmid-map-sequence-id-338587

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  15. pGEX-CS-MjPiwi_G

    Plasmid
    #146384
    Purpose
    Bacterial Expression of MjPiwi
    Depositor
    Elisa Izaurralde
    Insert
    MjPiwi
    Expression
    Bacterial
    Mutation
    one missense mutation C490A (H163N) compared to t…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146384-plasmid-map-sequence-id-288829

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  16. pcDNA3.1-HA-Bax-mito

    Plasmid
    #191943
    Purpose
    Expression of Bax and mitochodrial targeting sequence.
    Depositor
    Luca Scorrano
    Insert
    Bax-mito
    Expression
    Mammalian
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    191943-plasmid-map-sequence-id-379566

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  17. pUPD2 GFP (GB2237)

    Plasmid
    #160591
    Purpose
    GFP coding sequence.
    Depositor
    Diego Orzaez
    Insert
    GFP
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160591-plasmid-map-sequence-id-322075

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  18. pGGD_RT_biChromium

    Plasmid
    #136974
    Purpose
    Goldengate/Greengate C-tag module, containing a dual fluorescent marker based on the P2A peptide
    Depositor
    Klaus Theres
    Insert
    P2A sequence
    Expression
    Bacterial
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136974-plasmid-map-sequence-id-270595

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  19. pDIV131

    Plasmid
    #247965
    Purpose
    Cloning vector containing A-PacI-Nt.BbvcI cassette-B sequences compatible with the DIVERSIFY gene landing platform
    Depositor
    Uffe Mortensen
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    247965-plasmid-map-sequence-id-501719

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Showing: 3661 - 3680 of 23601 results