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MAC3-CDK8

Plasmid

#222609

Purpose

Expression of MAC3-tagged CDK8

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

CDK8 (CDK8 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEY131

Plasmid

#223478

Purpose

srx-105 fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

srx-105 (srx-105 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY137

Plasmid

#223484

Purpose

tax-2 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tax-2 (tax-2 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY138

Plasmid

#223485

Purpose

tol-1(3k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tol-1(3k) (tol-1 Nematode)

Expression

Worm

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEY144

Plasmid

#223491

Purpose

unc-39(3k) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-39 (3k) (unc-39 Nematode)

Expression

Worm

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR092-L31

Plasmid

#185045

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31 coding region, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR112

Plasmid

#185048

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region K2A mutation, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31p

Expression

Bacterial

Mutation

K2A amino acid mutation

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR113

Plasmid

#185049

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region H6A mutation, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31p

Expression

Bacterial

Mutation

H6A amino acid mutaitons

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR114

Plasmid

#185050

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region E8A mutation, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31p

Expression

Bacterial

Mutation

E8A amino acid mutation

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR120

Plasmid

#185041

Purpose

J23108 promoter, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Expression

Bacterial

Mutation

Only first 90 nt of rpmE coding present

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR089-L36p

Plasmid

#185042

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L36p coding region, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L36p

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR090-L36

Plasmid

#185043

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L36 coding region, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L36

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR091-L31p

Plasmid

#185044

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31p

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_EF-Fld

Plasmid

#202181

Purpose

Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Fld terminator

Use

Synthetic Biology

Mutation

None

Available Since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-D-Topo-kcnk17

Plasmid

#208999

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk17 (kcnk17 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk5a

Plasmid

#208988

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk5a (kcnk5a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk6

Plasmid

#208990

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk6 (kcnk6 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk9

Plasmid

#208992

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk9 (kcnk9 Zebrafish)

Use

Cloning

Mutation

Without STOP codon

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk13a

Plasmid

#208996

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk13a (kcnk13a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_EF-P49

Plasmid

#202176

Purpose

Level 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

P49 terminator

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 19, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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