We narrowed to 13,136 results for: BASE

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pCP5

Plasmid

#35397

Depositor

Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Type

Empty backbone

Use

Reporter

Expression

Yeast

Promoter

GDP promoter

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1F74G-FLAG

Plasmid

#189727

Purpose

Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1 F74G -FLAG

Plasmid

#189728

Purpose

Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

HTA1

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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CaYin1

Plasmid

#33067

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1

Tags

6 His tag

Expression

Bacterial

Promoter

ARA promoter

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only

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TALED_Left-stuffer-1397C-UGI

Plasmid

#187168

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-1397C-UGI

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFUS_A30A

Plasmid

#31029

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pET20b-GpNLuc-6xHis

Plasmid

#135932

Purpose

Expresses the GpNLuc LumiFluor in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

GpNLuc LumiFluor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-THT III

Plasmid

#55791

Purpose

GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistance

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Type

Empty backbone

Use

RNAi; Gateway

Expression

Mammalian

Promoter

modified H1 RNA gene

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry_LD 0_CS G_tTA-BFP

Plasmid

#58856

Purpose

MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pMC-Ura-Cen-lvl1

Plasmid

#176182

Purpose

preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-X2

Plasmid

#176152

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-X3

Plasmid

#176153

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI1

Plasmid

#176155

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI5

Plasmid

#176158

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XII2

Plasmid

#176160

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101107

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pUC18-iSpinach

Plasmid

#186053

Purpose

template for co-transcriptional RNA folding assays

Depositor

Markus Wahl

Article

Huang et al Mol Cell. 2020 Sep 17;79(6):1024-1036.e5

Insert

T7A1-rrnG-iSpinach

Tags

no

Expression

Bacterial

Promoter

T7A1

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJin-264

Plasmid

#125717

Purpose

ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19

Depositor

Bryan Dickinson

Article

Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

Insert

PT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI

Expression

Mammalian

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pL8_3xG34-DCAF8L2_X1

Plasmid

#191519

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG34-DCAF8L2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pL5_3xG22-DCAF8L2_X1

Plasmid

#191516

Purpose

Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE 3xG22-DCAF8L2 X1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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