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pRS413-TEF1pr-PMA1-FAPoptim

Plasmid

#221122

Purpose

FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

PMA1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC7-FAPoptim

Plasmid

#221124

Purpose

FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC7-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-VPH1-FAPoptim

Plasmid

#221125

Purpose

FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

VPH1-FAPoptim

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAG426

Plasmid

#226734

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

RHOT1 (RHOT1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4

Plasmid

#200230

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.5

Plasmid

#200232

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis Nav1.5

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4-N432T

Plasmid

#200284

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Mutation

N432T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4-N830T

Plasmid

#200285

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Mutation

N830T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4-N11306T

Plasmid

#200286

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Mutation

N11306T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4-N1609T

Plasmid

#200287

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Mutation

N1609T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pum NaV1.4-N1609A

Plasmid

#200288

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Pitohui uropygialis meridionalis NaV1.4

Expression

Mammalian

Mutation

N1609A

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Pt NaV1.4-N1600T

Plasmid

#200289

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Phyllobates terriblis NaV1.4 – muscle channel

Expression

Mammalian

Mutation

N1600T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Dt NaV1.4-N1600T

Plasmid

#200308

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Dendrobates tinctorius NaV1.4 – muscle channel

Expression

Mammalian

Mutation

N1600T

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5

Plasmid

#166163

Purpose

Gateway entry vector for N. furzeriDDX5

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Use

Gateway entry vector

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-DDX5(kf)-SNAP

Plasmid

#166152

Purpose

Expression of N. furzeri DDX5 in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Tags

His10 and SNAP

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-EGFP

Plasmid

#166137

Purpose

EGFP expression under the control of the GPD promoter yeast

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

EGFP

Expression

Yeast

Promoter

GDP

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-mRuby3

Plasmid

#166138

Purpose

mRuby3 expression under control of the GPD promoter in yeast

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

mRuby3

Expression

Yeast

Promoter

GDP

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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mApple-1xHp

Plasmid

#214409

Purpose

Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

Spastin aa 43-92 (SPAST Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-GSDMD_C39A-Myc

Plasmid

#201167

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Mutation

C39A

Promoter

MESV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEXPA10-NLS-3xmCitrine

Plasmid

#192523

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

EXPANSIN A10 (EXPA10 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

EXPA10

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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