We narrowed to 5,112 results for: mcs

1. CRISPRoff-mScarletI

   Plasmid

   #217783

   Purpose

   Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
   

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

   Plasmid

   #172910

   Purpose

   Mammalian expression of voltage sensor; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2-ST
   

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

   Plasmid

   #183080

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Mutation

   -124C>T promoter, Exon 1 C7C (C21>T21) sile…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

   Plasmid

   #183081

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Mutation

   -146C>T promoter, Exon 1 C7C (C21>T21) sile…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES2-smGFP-Kv2.1

   Plasmid

   #131709

   Purpose

   Expresses Kv2.1 in mammalian cells

   Depositor

   Jennifer Kearney

   Article

   Kang et al Ann Neurol. 2019 Dec;86(6):899-912. doi:

   Insert

   KCNB1 (KCNB1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEH_AAV_TERT_prom_C7C_1.8kb

   Plasmid

   #183079

   Purpose

   rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

   Depositor

   Aviv Regev

   Article

   Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

   Insert

   TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
   

   Use

   AAV; Homologous recombination donor template

   Mutation

   Wildtype promoter, Exon 1 C7C (C21>T21) silent…

   Promoter

   None for primary insert (Though partial TERT prom…

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-CAG-VARNAM A122D WPRE-bGH-polyA

   Plasmid

   #180486

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   VARNAM A122D
   

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.23-hs737.1

   Plasmid

   #222473

   Purpose

   Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 1). Variant 1 is chr10:128568698-A-G where the information is chromosome:positionInB38-RefAllele-AltAllele.

   Depositor

   Tychele Turner

   Article

   Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

   Insert

   hs737 enhancer sequence (sequence containing variant 1) (LOC110120928 Human)
   

   Use

   Luciferase

   Mutation

   Variant 1 is chr10:128568698-A-G where the inform…

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.23-hs737.3

   Plasmid

   #222475

   Purpose

   Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 3). Variant 3 is chr10:128569437-G-A where the information is chromosome:positionInB38-RefAllele-AltAllele.

   Depositor

   Tychele Turner

   Article

   Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

   Insert

   hs737 enhancer sequence (sequence containing variant 3) (LOC110120928 Human)
   

   Use

   Luciferase

   Mutation

   Variant 3 is chr10:128569437-G-A where the inform…

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.23-hs737.2

    Plasmid

    #222474

    Purpose

    Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 2). Variant 2 is chr10:128569418-T-C where the information is chromosome:positionInB38-RefAllele-AltAllele.

    Depositor

    Tychele Turner

    Article

    Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

    Insert

    hs737 enhancer sequence (sequence containing variant 2) (LOC110120928 Human)
    

    Use

    Luciferase

    Mutation

    Variant 2 is chr10:128569418-T-C where the inform…

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

    Plasmid

    #183078

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    V600E (T>A), S607S (TCC>AGT)

    Promoter

    None for primary insert. PGK for puromycin resist…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. t1-405(151-154AAAA)

    Plasmid

    #166131

    Purpose

    Expression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    T1head1-405(151-154AAAA) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    aa1-405 only. Contains 4 amino acid substitutions…

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. C321.deltaA (Isaacs lab)

    Bacterial Strain

    #73581

    Purpose

    Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Bacterial Resistance

    Ampicillin

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV Retrograde)

    Viral Prep

    #83898-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2 fused to mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    mDlx

    Tags

    mCherry

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-mDlx-GFP-Fishell-1 (AAV PHP.eB)

    Viral Prep

    #83900-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    mDlx

    Tags

    GFP

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-mDlx-GFP-Fishell-1 (AAV Retrograde)

    Viral Prep

    #83900-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    mDlx

    Tags

    GFP

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV Retrograde)

    Viral Prep

    #83896-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    hDlx

    Tags

    dTomato (physically separate, not a fusion protein)

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-hDlx-Flex-dTomato-Fishell_7 (AAV Retrograde)

    Viral Prep

    #83894-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hDlx-Flex-dTomato-Fishell_7 (#83894). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-dTomato-Fishell_7 plasmid DNA. hDlx-driven, Cre-dependent expression of dTomato. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    hDlx

    Tags

    dTomato (Cre-dependent)

    Available Since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-hDlx-Flex-GFP-Fishell_6 (AAV Retrograde)

    Viral Prep

    #83895-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hDlx-Flex-GFP-Fishell_6 (#83895). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-GFP-Fishell_6 plasmid DNA. mDlx-driven, Cre-dependent expression of GFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    hDlx

    Tags

    GFP

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV Retrograde)

    Viral Prep

    #83897-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    hDlx

    Tags

    dTomato

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only