We narrowed to 11,768 results for: sgrna

1. lentiCRISPRv2_ZsG gRNA_2

   Plasmid

   #192686

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPRv2_ZsG gRNA_3

   Plasmid

   #192687

   Purpose

   Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA

   Depositor

   Jeffrey Miner

   Insert

   Cas9, ZsGreen targeting sgRNA #3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, U6

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJR98

   Plasmid

   #187239

   Purpose

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458 V3

   Plasmid

   #226957

   Purpose

   CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP3

   Plasmid

   #79875

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into thrC

   Depositor

   Jason Peters, Stanley Qi, Carol Gross

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP2

   Plasmid

   #79874

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into amyE

   Depositor

   Jason Peters, Stanley Qi, Carol Gross

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458 eSp(1.1) V3

   Plasmid

   #226963

   Purpose

   CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_ACTB CRISPIE

   Plasmid

   #172853

   Purpose

   Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Inserts

   Actb intron 1 sgRNA
   DRS-2 sgRNA
   CRISPIE designer exon (phase 0-0) encoding mEGFP
   mRuby3
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. SaCas9 GFP V3

   Plasmid

   #226962

   Purpose

   CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Pdg459-eSp(1.1) V3

    Plasmid

    #226965

    Purpose

    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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