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pSC-cUnaG

Plasmid

#207631

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-cUnaG

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSC-LPETG

Plasmid

#207629

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) with a C-terminal Sortase recognition sequence.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-LPETG

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHWBF09exp

Plasmid

#130833

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP2del4

Plasmid

#130837

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of terminal four amino acid residues

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP1delTM

Plasmid

#130841

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of transmembrane domain

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKG1delKASH

Plasmid

#130842

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of KASH domain

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP2del4ec

Plasmid

#130843

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Use

Unspecified

Tags

eGFP-FLAG-HA

Mutation

Deletion of terminal four amino acid residues

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP1del4ec

Plasmid

#130844

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Mutation

Deletion of terminal four amino acid residues

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP1delKASHec

Plasmid

#130845

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Mutation

Deletion of KASH domain, addition of three alanin…

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKG1delKASHec

Plasmid

#130846

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Mutation

Deletion of KASH domain

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHWBF07EC

Plasmid

#130828

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHWBF09EC

Plasmid

#130829

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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ECPK1D

Plasmid

#130830

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKG1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHWBF07exp

Plasmid

#130832

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Tags

eGFP-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_DE-Venus

Plasmid

#202157

Purpose

Level 0 plasmid containing a Venus fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Venus gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_DE-sfGFP

Plasmid

#202156

Purpose

Level 0 plasmid containing a sfGFP fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

sfGFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE_mTurquoise2

Plasmid

#202155

Purpose

Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mTurquoise2 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_DE-RFP1

Plasmid

#202154

Purpose

Level 0 plasmid containing a RFP1 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

RFP1 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_DE-mRuby3

Plasmid

#202153

Purpose

Level 0 plasmid containing a mRuby3 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mRuby3 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_DE-3xStop

Plasmid

#202151

Purpose

Level 0 plasmid containing 3 consecutive stop codons with DE overhangs used to build a level 1 constuct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

3x Stop codon

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_CD-eGFP

Plasmid

#202149

Purpose

Level 0 plasmid containing an eGFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

eGFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_CD-Venus

Plasmid

#202148

Purpose

Level 0 plasmid containing a Venus fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Venus gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_CD-sfGFP

Plasmid

#202147

Purpose

Level 0 plasmid containing a sfGFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

sfGFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_CD-mTurquoise2

Plasmid

#202146

Purpose

Level 0 plasmid containing a mTurquoise2 fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mTurquoise2 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_CD-mRFP1

Plasmid

#202145

Purpose

Level 0 plasmid containing a mRFP1 fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mRFP1 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_CD-eYGFP-uv

Plasmid

#202144

Purpose

Level 0 plasmid containing an eYGFP-uv fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

eYGFP-uv gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_CD-mcherry

Plasmid

#202143

Purpose

Level 0 plasmid containing a mCherry fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mCherry gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_CD-eCFP

Plasmid

#202142

Purpose

Level 0 plasmid containing an eCFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

eCFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_CD-roGFP

Plasmid

#202140

Purpose

Level 0 plasmid containing a roGFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

roGFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_CD-eYFP

Plasmid

#202139

Purpose

Level 0 plasmid containing an eYFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

eYFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_AF-spacer

Plasmid

#202130

Purpose

Level 0 plasmid containing a small non-coding spacer sequence with AF overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Spacer

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
FKBP-TalinRod-mEmerald

Plasmid

#202382

Purpose

FKBP12-Talin(434-2541)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

FKBP12-Talin(434-2541)-mEmerald

Expression

Mammalian

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-TalinC11-mEmerald

Plasmid

#202381

Purpose

FKBP12-Talin(1974-2541)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

FKBP12-Talin(1974-2541)-mEmerald

Expression

Mammalian

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-TalinN12-FB(wt)

Plasmid

#202385

Purpose

mApple-Talin(1-2299)-FRB

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

mApple-Talin(1-2299)-FRB

Expression

Mammalian

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-TalinN3-FRB(wt)

Plasmid

#202384

Purpose

mApple-Talin(1-911)-FRB

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

mApple-Talin(1-911)-FRB

Expression

Mammalian

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-TalinN10-FRB

Plasmid

#202380

Purpose

mApple-Talin(1-1973)-FRB

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

mApple-Talin(1-1973)-FRB

Expression

Mammalian

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE Myc-epitope donor ORF2

Plasmid

#200376

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
H5_fold-0_Elsa

Plasmid

#201825

Purpose

Expresses H5_fold-0_Elsa in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H5_fold-0_Elsa

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc

Plasmid

#199759

Purpose

Elongation reporter construct to quantify elongation duration of 4_CGA stalling

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

synYFP[TTG/AGA]-4xArg[CGA]-nLuc

Expression

Yeast

Promoter

TetO7

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-1067L

Plasmid

#200253

Purpose

pBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

6 gRNAs targeting Doublesex, Intersex, bTublin

Expression

Insect

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc

Plasmid

#199760

Purpose

Elongation reporter construct to quantify elongation duration of 6_CGA stalling

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

synYFP[TTG/AGA]-6xArg[CGA]-nLuc

Expression

Yeast

Promoter

TetO7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc

Plasmid

#199761

Purpose

Elongation reporter construct to quantify elongation duration of 6_AGA stalling

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

synYFP[TTG/AGA]-6xArg[AGA]-cLuc

Expression

Yeast

Promoter

TetO7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA28-pMa-PspCas13b crRNA-TS1

Plasmid

#199459

Purpose

U6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

TS1 crRNA

Use

CRISPR

Promoter

mouse U6

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SP_H6_Halo_L172Q_KDEL_pBABEpu

Plasmid

#183690

Purpose

Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(L172Q)

Use

Retroviral

Mutation

L72Q in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SP_H6_Halo_M21K_F86L_KDEL_pBABEpu

Plasmid

#183691

Purpose

Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(M21K_F86L)

Use

Retroviral

Mutation

M21K_F86L in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30_Halotag_K73T_Jdomain

Plasmid

#183693

Purpose

Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)-J domain

Expression

Bacterial

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p2621

Plasmid

#198144

Purpose

Epitope tagging of effector protein PipB2

Depositor

Michael Hensel

Article

Goser et al Nat Commun. 2023 Mar 4;14(1):1240. doi:

Insert

PpipB2 pipB2::M45 (STM2780 Salmonella enterica serovar Typhimurium)

Tags

M45

Expression

Bacterial

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p2643

Plasmid

#198145

Purpose

Epitope tagging of effector protein SseF

Depositor

Michael Hensel

Article

Goser et al Nat Commun. 2023 Mar 4;14(1):1240. doi:

Insert

PsseA::sscB sseF::HA (sseF Salmonella enterica serovar Typhimurium)

Tags

HA

Expression

Bacterial

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEYFP-N1-HsSmg6-mut-siRNAres_I

Plasmid

#146588

Purpose

Mammalian Expression of HsSmg6-mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al RNA. 2008 Dec . 14(12):2609-17.

Insert

HsSmg6-mut-siRNAres (SMG6 Human)

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pORE303N_CEN12Fuzzy3MYB

Plasmid

#194440

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12 and Fuzzy3MYB

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA array targeting CEN1, Fuzzy3MYB

Use

CRISPR

Expression

Plant

Promoter

MtU6

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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