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We narrowed to 60,801 results for: cloning

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Showing: 3741 - 3760 of 60801 results

  1. psBIG1e

    Plasmid
    #229961
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229961-plasmid-map-sequence-id-461266

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  2. psBIG1c

    Plasmid
    #229959
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229959-plasmid-map-sequence-id-461263

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  3. pnYC-NpM-HsNot10_25-707_AF

    Plasmid
    #148678
    Purpose
    Bacterial Expression of HsNot10_25-707
    Depositor
    Elisa Izaurralde
    Insert
    HsNot10_25-707 (CNOT10 Human)
    Expression
    Bacterial
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148678-plasmid-map-sequence-id-291126

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  4. p667-UBC-hcRed-V5_IDG-K

    Plasmid
    #135239
    Purpose
    Gateway destination clone of hcRed (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    hcRed-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135239-plasmid-map-sequence-id-269113

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  5. p663-UBC-V5-eGFP_IDG-K

    Plasmid
    #135265
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135265-plasmid-map-sequence-id-268086

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  6. pHAGE-CMV-Flag-hcRed_IDG-K

    Plasmid
    #135274
    Purpose
    Gateway destination clone of hcRed (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-hcRed
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    135274-plasmid-map-sequence-id-267146

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  8. TJP3

    Plasmid
    #25310
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TJP3:G508-L808:cH6 (TJP3 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids G508-L808
    Available Since
    April 4, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. Pf-FKBP-TPR

    Plasmid
    #25582
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pf-FKBP-TPR (FKBP35 Plasmodium Falciparum)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 128-304.
    Available Since
    Oct. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. SGF29 (3MEW)

    Plasmid
    #25320
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SGF29_23 (SGF29 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 129-291
    Available Since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

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  14. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

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  16. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  17. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid
    #138148
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NES
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138148-plasmid-map-sequence-id-318593

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  18. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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Showing: 3741 - 3760 of 60801 results