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Plasmid#125688PurposeGene knockout/in for actinomycetesDepositorInsertstreptomyces codon optimized spCas9, sgRNA casette, and ScaligD
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterermE*/tipAAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[sfGFP]
Plasmid#213902PurposeMariner transposon expressing sfGFP with kan selection casetteDepositorInsertmariner transposon with kan resistance and superfolder GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWY
Plasmid#112906PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGZ001
Plasmid#48868PurposeEmpty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.DepositorTypeEmpty backboneUseGolden gate compatible plant transformation vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141B-ge4.1
Plasmid#205144PurposeGuide RNA expression cassette harboring AtU3 promoter, ge4.1 5’ modification, and ge4.1 3’ modificationDepositorInsertgRNA cloning site
UseCRISPRTagsExpressionMutationPromoterAtU3Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGZ003
Plasmid#48869PurposeEmpty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.DepositorTypeEmpty backboneUseGolden gate compatible plant transformation vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCVII
Plasmid#162750PurposeMSCV backboneDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
DV4 VLPs pVRC8400
Plasmid#63164PurposeDV4 prM-E structural cassette in pVRC8400DepositorInsertDV4 prM-E
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
DV3 VLPs pVRC8400
Plasmid#63163PurposeDV3 prM-E structural cassette in pVRC8400DepositorInsertDV3 prM-E
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-Stuffer
Plasmid#106248PurposeDestination vector for U6::gRNA expression cassetteDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1662
Plasmid#112050PurposeC-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEUDepositorInsertsIAA17
TIR1
UseTagsHAExpressionYeastMutationonly amino acids 71-114PromoterADH1 and NoneAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLS.436
Plasmid#184953PurposeExpress -Eco1 RT and ncRNADepositorInsertEco1: ncRNA(wt) and RT
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWR
Plasmid#112905PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPAP004
Plasmid#160911PurposeE.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastorisDepositorInsertlacZ selection cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWC
Plasmid#112903PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWF
Plasmid#112904PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mWasabi.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSAC212
Plasmid#187912Purposepsac212-crispey-bar-ade2KODepositorInsertade2 KO CRISPEY cassette
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GW-HygroR
Plasmid#149610PurposeGateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.DepositorInsertGateway cassette (ccdb + Chlor.R.)
UseTagsExpressionInsectMutationPromoterAct5cAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_H2_beta_CM
Plasmid#184870PurposepSwap plasmid part with H2_beta homology arms and chloramphenicol cassetteDepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_17_pKD46.10
Plasmid#178917PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRE57-Ter
Plasmid#170635PurposeDNA donor for transpositionDepositorInsertMini-Tn6677, Ter cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5
Plasmid#61255PurposeCRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pE_Cre_lambdaCos
Plasmid#173785PurposeThe pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.DepositorInsertCre
UseCre/Lox and Synthetic BiologyTagsExpressionMutationS102Y and S279Y- please see depositor commentsPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLICER
Plasmid#197288PurposeConjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transferDepositorInsertI-SceI Endonuclease
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC574
Plasmid#127975PurposeAMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInserthygB (hygromycin resistance marker)
UseFungal expressionTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV-Floxed Luc
Plasmid#60622PurposeExpresses a conditional luciferase cassette dependent on Cre mediated recombinationDepositorInsertFloxed Luciferase
UseCre/Lox, Lentiviral, and LuciferaseTagsExpressionMutationPromoterhuman UbCAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0584
Plasmid#133471PurposeIntegration into fission yeast genome at ura4 locus with natMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_H2_beta_GM
Plasmid#184871PurposepSwap plasmid part with H2_beta homology arms and gentamycin cassetteDepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGGY001
Plasmid#48866PurposeEmpty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.DepositorTypeEmpty backboneUseGolden gate compatible plant transformation vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGY003
Plasmid#48867PurposeEmpty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.DepositorTypeEmpty backboneUseGolden gate compatible plant transformation vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only