We narrowed to 5,312 results for: pcDNA3.1(+)

1. pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

   Plasmid

   #188992

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Use

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   K109R, P245A, G328S, G381V, G429D

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 RL3Sc

   Plasmid

   #109365

   Purpose

   Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-ERK2 L198A L232A

   Plasmid

   #8978

   Depositor

   John Blenis

   Article

   Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   L198A, L232A

   Promoter

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3 PBD wt (Nigg AH12)

   Plasmid

   #41162

   Depositor

   Erich Nigg

   Article

   Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

   Insert

   PBD (PLK1 Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   Contains Plk1-PBD only (aa 326–603)

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-HCMVgL-FLAG (Merlin)

   Plasmid

   #136450

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein L (UL115 Human betaherpesvirus 5)
   

   Use

   Tags

   Short SGGSG linker and FLAG tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pER-eZinCh-2

   Plasmid

   #70009

   Purpose

   expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

   Depositor

   Maarten Merkx

   Article

   Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

   Insert

   ER-eZinCh-2
   

   Use

   Tags

   KDEL sequence and PPI signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dr-EGFR

   Plasmid

   #184004

   Purpose

   Dr-EGFR expression in mammalian cells

   Depositor

   Vladislav Verkhusha

   Article

   Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

   Insert

   Dr-EGFR (EGFR Human)
   

   Use

   Tags

   Deinococcus radiodurans bacterial phytochrome

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmyc-GFP-TNRC6A-NES-mut

   Plasmid

   #42001

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   F951A, F955A, I958A, F960A

   Promoter

   CMV

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. FKBP-CD28TMD-NTEVp(WT)

   Plasmid

   #137826

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(WT)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-YL1

    Plasmid

    #15333

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

    Insert

    transcription factor-like 1 (VPS72 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA_DiCre_60.F2

    Plasmid

    #60208

    Purpose

    Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

    Depositor

    Jean-Paul Herman

    Article

    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

    Insert

    Cre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
    

    Use

    Tags

    FRAP and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    amino acids 2021-2112 of FRAP with a T2098L mutat…

    Promoter

    CMV

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. psgRNA2.02-PP7-ccdB

    Plasmid

    #99912

    Purpose

    Basic cloning vector for the expression of PP7 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-NES-ZapCmR2

    Plasmid

    #59015

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

14. mGFAP-Halo

    Plasmid

    #169474

    Purpose

    the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    GFAP (Gfap Mouse)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Changed R 236 to H

    Promoter

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. psgRNA2.02-MS2-ccdB

    Plasmid

    #99911

    Purpose

    Basic cloning vector for the expression of MS2 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Bongwoori

    Plasmid

    #63720

    Purpose

    Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    Bongwoori
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGP-CMV-jGCaMP8f F366H

    Plasmid

    #204141

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Use

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    CMV

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

    Plasmid

    #188993

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    D201G, A258T, K327R, G328S, G381V, G429D, T450A

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmyc-GFP-TNRC6A-NLS-mut

    Plasmid

    #42000

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    R930A, R931A, R932A, R934A

    Promoter

    CMV

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSSA-PMG (pXK-1649)

    Plasmid

    #208857

    Purpose

    SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.

    Depositor

    Kun Xu

    Article

    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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