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MSCV-Puro-FH-AGO2-S824A

Plasmid

#91987

Purpose

Expresses FLAG-HA-AGO2 (S824A)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-T830A

Plasmid

#91988

Purpose

Expresses FLAG-HA-AGO2 (T830A)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

T830A

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S831A

Plasmid

#91989

Purpose

Expresses FLAG-HA-AGO2 (S831A)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S831A

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S834A

Plasmid

#91990

Purpose

Expresses FLAG-HA-AGO2 (S834A)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S834A

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S828A

Plasmid

#91991

Purpose

Expresses FLAG-HA-AGO2 (S828A)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828A

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E

Plasmid

#91994

Purpose

Expresses FLAG-HA-AGO2 (S824E)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S828E

Plasmid

#91995

Purpose

Expresses FLAG-HA-AGO2 (S828E)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828E

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

Plasmid

#92433

Purpose

expresses Cas9 and gRNA targeting PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Publication

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

CRISPR

Expression

Mammalian

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad3

Plasmid

#89828

Purpose

Knockdown human Smad3 expression in human mammalian cells

Depositor

Shengyu Yang

Publication

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD3 shRNA (SMAD3 Human)

Use

Retroviral

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v2 puro

Plasmid

#87793

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Publication

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP7.v1 puro

Plasmid

#87794

Purpose

Expresses an inducible short hairpin targeting human MKP7 sequence

Depositor

Kevin Janes

Publication

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP7 (DUSP16 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v1 puro

Plasmid

#87792

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Publication

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-Puro-DUS2L-PSKH1

Plasmid

#69472

Purpose

Expresses DUS2L-PSKH1 fusion gene

Depositor

Axel Hillmer

Publication

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

DUS2L-PSKH1 (PSKH1 Human)

Use

Retroviral

Tags

HA TAG

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-LRTM2

Plasmid

#69237

Purpose

U6 driven SpCas9 sgRNA expression for LRTM2 site

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

LRTM2 sgRNA (LRTM2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-mRuby-WDR34

Plasmid

#66599

Purpose

Expresses WDR34 as a fusion with monomeric mRuby

Depositor

David Stephens

Publication

Asante et al J Cell Sci. 2014 Nov 1;127(21):4774-87.

Insert

WDR34 (WDR34 Human)

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pMKO.1-puro/hFOXH1 shRNA3

Plasmid

#59308

Purpose

Knockdown of human FOXH1

Depositor

Shinya Yamanaka

Publication

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

hFOXH1 shRNA (FOXH1 Human)

Use

RNAi and Retroviral

Promoter

hU6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro U6 sgRNA SOX17 -91

Plasmid

#50923

Purpose

U6 driven sgRNA targeting Sox17 -91 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17 -91 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLX-V5-TRAF2 S102A-puro

Plasmid

#44113

Depositor

William Hahn

Publication

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Mutation

Serine 102 to Alanine

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only
pBabe-Puro-RalA-S11A

Plasmid

#15253

Depositor

William Hahn

Publication

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

v-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

serine 11 to alanine

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pBABE puro rat IRS-3

Plasmid

#11362

Depositor

Ronald Kahn

Publication

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-3 (Irs3 Rat)

Use

Retroviral

Expression

Mammalian

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only