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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,206 results for: sgRNA

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Showing: 3761 - 3780 of 9206 results

  1. pRDA_835

    Plasmid
    #245327
    Purpose
    Cas9 CRISPRko positive control guide; targets CD59
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD59 (CD59 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245327-plasmid-map-sequence-id-488049

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  2. ttAtCas12a+int

    Plasmid
    #182384
    Purpose
    Gives very high editing efficiency in barley. Also works in Brassica oleracea
    Depositor
    Wendy Harwood
    Publication
    Lawrenson et al bioRxiv 2022.04.28.489853
    Insert
    LbCas12a coding sequence with D156R and Arabidopsis introns
    Use
    CRISPR
    Mutation
    D156R + 8 Arabidopsis introns added
    Promoter
    None
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182384-plasmid-map-sequence-id-361472

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  6. pK2-J11

    Plasmid
    #218224
    Purpose
    Terminates AtU6-promoter driven sgRNA array with two guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    218224-plasmid-map-sequence-id-431992

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  7. pK4-J11

    Plasmid
    #218225
    Purpose
    Terminates AtU6-promoter driven sgRNA array with four guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    218225-plasmid-map-sequence-id-431986

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  8. pK6-J11

    Plasmid
    #218226
    Purpose
    Terminates AtU6-promoter driven sgRNA array with tsix guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    218226-plasmid-map-sequence-id-431985

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  9. pK8-J11

    Plasmid
    #218227
    Purpose
    Terminates AtU6-promoter driven sgRNA array with eight guides, MoClo Compatible
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    218227-plasmid-map-sequence-id-431982

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  10. pK2-K3

    Plasmid
    #218221
    Purpose
    Fuses Guides 1+2 to guides 3+4 in an of AtU6-promter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    218221-plasmid-map-sequence-id-431988

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  11. pK4-K5

    Plasmid
    #218222
    Purpose
    Fuses Guides 3+4 to guides 6+6 in an of AtU6-promter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    218222-plasmid-map-sequence-id-431983

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  12. pK6-K7

    Plasmid
    #218223
    Purpose
    Fuses Guides 5+6 to guides 7+8 in an of AtU6-promter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    linker sequence
    Use
    CRISPR
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    218223-plasmid-map-sequence-id-431979

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  15. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  17. pTJK459

    Plasmid
    #138008
    Purpose
    Empty sgRNA expression vector for expression of sgRNA for Sp Cas9
    Depositor
    Tami Kingsbury
    Type
    Empty backbone
    Use
    Lentiviral
    Mutation
    U-A flip and hairpin extension: cgtttAagagctaTGCT…
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    138008-plasmid-map-sequence-id-275002

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  19. B-SpCas9

    Plasmid
    #126760
    Purpose
    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    amino acids 1005-1013 replaced with two glycine
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126760-plasmid-map-sequence-id-261519

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  20. B-eSpCas9

    Plasmid
    #126761
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126761-plasmid-map-sequence-id-259548

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Showing: 3761 - 3780 of 9206 results