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Plasmid#223723PurposeExpression of recombinant protein for purificationDepositorInsertEGFP, not monomeric (has A207)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVT-Bxb1-I87L, A369P, E434G
Plasmid#232750PurposeIVT template for making modRNA encoding mutant Bxb1modRNA (I87L, A369P, E434G)DepositorInsertBxb1
UseIn vitro transcriptionTagsHA and SV40 NLSExpressionMutationI87L, A369P, E434GPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_CAG-ITPKA-mScarlet-I
Plasmid#229778PurposeBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporterDepositorInsertmScarlet-I
UseSynthetic BiologyTagsITPKAExpressionMammalianMutationPromoterCAGAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Nat-ADE2
Plasmid#232106PurposeExpresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterZ3Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Hyg-ADE2
Plasmid#232107PurposeExpresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterZ3Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
SUPV3L1-HA WT
Plasmid#232348PurposeExpresses human SUPV3L1/SUV3 in mammalian cells with HA tagDepositorInsertSUPV3L1 (SUPV3L1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SUPV3L1-HA G207V
Plasmid#232349PurposeExpresses human SUPV3L1/SUV3 (G207V) in mammalian cells with HA tagDepositorInsertSUPV3L1 (SUPV3L1 Human)
UseTagsHAExpressionMammalianMutationG207VPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_mScarlet_Convergent
Plasmid#229790PurposeBxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent
Plasmid#229800PurposeBxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1aDepositorInsertmGreenLantern; mRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1a and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_mScarlet_Divergent
Plasmid#229791PurposeBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCEFL-HAx2-TEADiv2
Plasmid#228873PurposeExpression of optimized TEAD inhibitor (TEADiv2): 2xHA-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signalDepositorInsertTEADiv2
UseTags2xHAExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCEFL-EGFP-TEADiv2
Plasmid#228872PurposeExpression of optimized TEAD inhibitor (TEADiv2): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signalDepositorInsertTEADiv2
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHPHS926
Plasmid#228237PurposeBxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin NMD reporter with premature termination codon for integration into Bxb1 attP_GA landing padDepositorInsertsmCherry
Beta globin
UseTags3xFLAG, HA, and PuroRExpressionMammalianMutationPTC39PromoterTRE3GV and noneAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
OSCA1.2-K435L-L438K-peGFP-N1
Plasmid#228587PurposeExpresses OSCA1.2-K435L-L438K-eGFP in mammalian cellsDepositorInserthyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
UseTagsExpressionMammalianMutationK435L, L438KPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Plagl2
Plasmid#228061Purposetransiently express mouse Plagl2DepositorInsertPlagl2 (Plagl2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y115S/Y132S/Y139S
Plasmid#216408PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutationsDepositorInsertEfg1-ΔDBD-Y115S/Y132S/Y139S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 115, 132 and 139 to Serine, and …PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_HD_160-223AA
Plasmid#221489PurposeNKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 1-159AA N-terminal and 224-371AA C-te…PromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP670-C1-Nir1-LNS2
Plasmid#220085Purposehigh affinity PA biosensor with far-red fluorophoreDepositorInsertPITPNM3 (PITPNM3 Human)
UseTagsiRFP670-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL475
Plasmid#219503PurposeExpress Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in transDepositorInsertAde2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-N1-Nir1-613-630
Plasmid#220088PurposeN-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PADepositorInsertPITPNM3 (PITPNM3 Human)
UseTagsGGSGG-NeonGreenExpressionMammalianMutationamino acids 613-630PromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P/Q443P
Plasmid#216402PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56 and Glutamine 443 to Proli…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y4S/Y8S/Y16S
Plasmid#216405PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutationsDepositorInsertEfg1-ΔDBD-Y4S/Y8S/Y16S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 4, 8 and 16 to Serine, and delet…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y64S/Y66S/Y69S
Plasmid#216406PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutationsDepositorInsertEfg1-ΔDBD-Y64S/Y66S/Y69S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 64, 66 and 69 to Serine, and del…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y101S/Y104S/Y106S
Plasmid#216407PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutationsDepositorInsertEfg1-ΔDBD-Y101S/Y104S/Y106S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 101, 104 and 106 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y401S/Y404S/Y409S
Plasmid#216409PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutationsDepositorInsertEfg1-ΔDBD-Y401S/Y404S/Y409S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 401, 404 and 409 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y450S/Y452S/Y456S
Plasmid#216410PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutationsDepositorInsertEfg1-ΔDBD-Y450S/Y452S/Y456S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 450, 452 and 456 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y478S/Y482S/Y484S
Plasmid#216411PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutationsDepositorInsertEfg1-ΔDBD-Y478S/Y482S/Y484S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 478, 482 and 484 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
1114H
Plasmid#200640PurposeAn. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile malesDepositorInsertgRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-Hyg
Plasmid#232091PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRTagsExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only