Addgene

We narrowed to 654 results for: MIM

Showing: 361 - 380 of 654 results

Results per page:

Sort results by:

pEGFP-Flag-stm2585

Plasmid

#174397

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Use

Tags

EGFP and Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWSK129-stm2585_S130A

Plasmid

#174403

Purpose

Bacterial expression of sarA with its GSK3-binding SxxxSP motif mutated to SxxxAP.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Use

Tags

Expression

Bacterial

Mutation

sarA with S130A mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWSK129-stm2585_Y167F

Plasmid

#174402

Purpose

Bacterial expression of sarA with its STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Use

Tags

Expression

Bacterial

Mutation

sarA with Y167F mutation

Promoter

Endogenous

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Flag-gp130dimer-stm2585

Plasmid

#174395

Purpose

Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

IL6ST cytosolic domain and sarA chimera

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Flag-stm2585

Plasmid

#174391

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tag

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PymeACR1

Plasmid

#153031

Purpose

Prasinophyte anion-conducting channelrhodopsin. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

Insert

PymeACR1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV (+ enhancer)

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-AthHEN1-CM

Plasmid

#104981

Purpose

Expression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Use

Tags

FLAG Myc

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK381

Plasmid

#71573

Purpose

Produces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)

Depositor

T. J. Kappock

Article

Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

Insert

citrate synthase

Use

Tags

Expression

Bacterial

Mutation

changes aspartate-317 to glycine

Promoter

recA

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK382

Plasmid

#71574

Purpose

Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)

Depositor

T. J. Kappock

Article

Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

Insert

citrate synthase

Use

Tags

Expression

Bacterial

Mutation

changes aspartate-317 to asparagine; silent mutat…

Promoter

recA

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-7 asyn WT

Plasmid

#36046

Depositor

Hilal Lashuel

Article

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPRi(v2)-Blast

Plasmid

#170068

Purpose

Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

KRAB-dCas9-MeCP2

Use

CRISPR and Lentiviral

Tags

2A and 2xHA tag

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRXIP TMEM41B-GFP

Plasmid

#117414

Purpose

Expresses TMEM41B tagged with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

TMEM41B (TMEM41B Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRXIP TMEM41B-3xFLAG

Plasmid

#117415

Purpose

Expresses TMEM41B tagged with 3xFLAG in mammalian cells

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

TMEM41B (TMEM41B Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-mCherry

Plasmid

#75161

Purpose

Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
NO- DSD Sensor 2

Plasmid

#199786

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO- DSD Sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TS MS-Sensor

Plasmid

#199788

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

TS MS-Sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TT MS-Sensor

Plasmid

#199789

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

TT MS-Sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXsIP GFP-VMP1

Plasmid

#117417

Purpose

Expresses VMP1 tagged with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

VMP1 (VMP1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV asyn WT

Plasmid

#36055

Depositor

Hilal Lashuel

Article

Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

Insert

alpha-synuclein WT (SNCA Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pPEP-TEV cNup98 (1-498)

Plasmid

#38037

Depositor

Roderick Lim

Article

Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

Insert

Nucleoporin 98 (NUP98 Human)

Use

Tags

His-tag, 3Cysteine

Expression

Bacterial

Mutation

amino acids 1-498

Promoter

T7

Available since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge