We narrowed to 814 results for: Pef1

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Integrin alpha5-AHCys

Plasmid

#27313

Depositor

Timothy Springer

Article

Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

Insert

integrin alpha 5 (ITGA5 Human)

Expression

Mammalian

Mutation

integrin alpha5 (1-954) + AHCys

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only
PM PKA Reporter

Plasmid

#104551

Purpose

Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Lyn-mTurquoise2-VASP 148 - 164

Tags

mTurquoise 2, HA tag

Expression

Mammalian

Promoter

EF1 alpha

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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Integrin alpha5(1-623)-AHCys

Plasmid

#27301

Depositor

Timothy Springer

Article

Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

Insert

integrin alpha 5 (ITGA5 Human)

Expression

Mammalian

Mutation

alpha 5(1-623)+GG+TEV+GGEN+AHCys

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only
OMM PKA Reporter

Plasmid

#104550

Purpose

Expresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Tom20 - mTurquoise2 - VASP 148-164

Tags

mTurqouise2

Expression

Mammalian

Promoter

EF1 alpha

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one_Bip gRNA1

Plasmid

#183696

Purpose

Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

gRNA targeting chinese hamster Bip (Hspa5)

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDD2604

Plasmid

#175088

Purpose

Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

S644A

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDD2601

Plasmid

#175085

Purpose

Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions S47A, S65A, T75A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

S47A, S65A, T75A

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2602

Plasmid

#175086

Purpose

Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

T424A, T438A

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2603

Plasmid

#175087

Purpose

Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

T504A, T508A

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPRa_all-in-one_Bip gRNA1

Plasmid

#183696

Purpose

Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

gRNA targeting chinese hamster Bip (Hspa5)

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2604

Plasmid

#175088

Purpose

Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

S644A

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2601

Plasmid

#175085

Purpose

Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions S47A, S65A, T75A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

S47A, S65A, T75A

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2602

Plasmid

#175086

Purpose

Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

T424A, T438A

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2603

Plasmid

#175087

Purpose

Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

T504A, T508A

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHPHS800

Plasmid

#228240

Purpose

Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Insert

Cas9

Tags

Hygromycin phosphotransferase

Expression

Mammalian

Promoter

none

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR

Plasmid

#160046

Purpose

For expressing the full paradoxical circuit

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Inserts

osTIR1
iaaH
iCasp9
BlastR

Use

Synthetic Biology

Tags

mAID, mCherry, and mGFPmut3

Expression

Mammalian

Promoter

no promoter, T2A is used and pEF1s

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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Dom neg CstF64

Plasmid

#127250

Purpose

Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

CstF64 delta 282 (CSTF2 Human)

Use

Other

Expression

Mammalian

Mutation

insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

Promoter

pEF1a

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR

Plasmid

#160046

Purpose

For expressing the full paradoxical circuit

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Inserts

osTIR1
iaaH
iCasp9
BlastR

Use

Synthetic Biology

Tags

mAID, mCherry, and mGFPmut3

Expression

Mammalian

Promoter

no promoter, T2A is used and pEF1s

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge