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We narrowed to 10,767 results for: SEC

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Showing: 361 - 380 of 10767 results

  1. pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

    Plasmid
    #154838
    Purpose
    Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection
    Depositor
    Patrick Phillips
    Article
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    synthetic guide::∆HYGR::unc-54 3' UTR
    Use
    CRISPR and Cre/Lox
    Expression
    Worm
    Mutation
    ∆HYGR is promoterless and encodes aa 60-341
    Available Since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154838-plasmid-map-sequence-id-307904

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  4. PSB-CMV-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-Sec61B

    Plasmid
    #250913
    Purpose
    Dual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS1-2 and EGFP tagged human Sec 61B in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Sec61B (SEC61B, ARFGAP1 Human)
    Tags
    EGFP on Sec61b and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 192–304 only
    Promoter
    CMV
    Available Since
    Feb. 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    250913-plasmid-map-sequence-id-498650

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  5. PSB-CMV-Puro ArfGAP1_ALPS1-mkate2-3NLS-P2A-EGFP-Sec61B

    Plasmid
    #250912
    Purpose
    Dual color expression of mKate2-3NLS tagged rat ARFGAP1 ALPS1 and EGFP tagged human Sec 61B in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Sec61B (Arfgap1, SEC61B Human, Rat)
    Tags
    EGFP on Sec61B and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 196–234 only
    Promoter
    CMV
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250912-plasmid-map-sequence-id-498488

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  7. pSB-CMV-MCS-Puro mCherry-Sec 61B -P2A-EGFP-LMNB1

    Plasmid
    #250911
    Purpose
    Dual color expression of mCherry tagged human Sec 61B and EGFP tagged zebrafish LaminB1 in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    mCherry-Sec61B-P2A-EGFP-LMNB1 (SEC61B, lmnb1 Human, Zebrafish)
    Tags
    EGFP on LMNB1 and mCherry on Sec61b
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250911-plasmid-map-sequence-id-501821

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  8. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Assembly of transcription units for protein secretion in the yeasts Saccharomyces cerevisiae and Pichia pastoris.
    Depositor
    Sylvestre Marillonnet
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  9. MoClo Yeast Secretion and Display (YSD) Toolkit

    Plasmid Kit
    #1000000240
    Purpose
    Collection of plasmids to optimize secretion and cell surface display of proteins of interest in yeast.
    Depositor
    Paul Young
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  10. MoClo Yeast Secretion Biosensor (YSB) Toolkit

    Plasmid Kit
    #1000000268
    Purpose
    Library of plasmids containing a variety of signal peptides, C-tags, and alpha-mating factor tags for use in protein secretion optimization in yeast.
    Depositor
    Joseph Brock
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only

  11. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid Kit
    #1000000109
    Purpose
    A collection of C. elegans plasmids used for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC), based on the SapTrap CRISPR/Cas Toolkit.
    Depositor
    Bob Goldstein
    Application
    Genome Editing
    Vector Type
    Worm Expression
    Editing Type
    CRISPR
    Available Since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  12. pDD288

    Plasmid
    #137017
    Purpose
    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mNG-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mNG
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137017-plasmid-map-sequence-id-274039

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  13. pDD291

    Plasmid
    #137019
    Purpose
    mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mKate2-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mKate2
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137019-plasmid-map-sequence-id-274037

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  14. pDD316

    Plasmid
    #137020
    Purpose
    mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mTurq2-GLO^SEC^2xHA + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    2xHA and C. elegans GLO-optimized mTurq2
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137020-plasmid-map-sequence-id-274032

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  15. pDD289

    Plasmid
    #137018
    Purpose
    GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    GFP-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized GFP
    Expression
    Worm
    Available Since
    April 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137018-plasmid-map-sequence-id-291422

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  16. pUA97

    Plasmid
    #69340
    Purpose
    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
    Depositor
    Oliver Hobert
    Insert
    ceBFP SEC 3xFlag
    Tags
    3xFlag and ceBFP (mTagBFP)
    Expression
    Worm
    Mutation
    sqt-1(e1350)
    Available Since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69340-plasmid-map-sequence-id-133241

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  17. pDD346

    Plasmid
    #133311
    Purpose
    SapTrap mNG-C1 donor
    Depositor
    Daniel Dickinson
    Insert
    C. elegans codon-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans codon-optimized mNeonGreen
    Expression
    Worm
    Promoter
    None
    Available Since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    133311-plasmid-map-sequence-id-263341

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  18. pDD373

    Plasmid
    #137021
    Purpose
    SapTrap GFP-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized GFP
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized GFP
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137021-plasmid-map-sequence-id-274046

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  19. pDD400

    Plasmid
    #137024
    Purpose
    SapTrap mNG-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mNeonGreen
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137024-plasmid-map-sequence-id-274031

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  20. pJJR81

    Plasmid
    #75029
    Purpose
    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Mike Boxem
    Insert
    tagBFP2^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized tagBFP2
    Expression
    Worm
    Available Since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75029-plasmid-map-sequence-id-141917

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Showing: 361 - 380 of 10767 results