Addgene

We narrowed to 723 results for: Tcta

Showing: 361 - 380 of 723 results

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CSNK2B H4.4 gRNA

Plasmid

#90643

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK2B

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CSNK2B (Guide Designation H4.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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CDC25C B6.2 gRNA

Plasmid

#90591

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC25C

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDC25C (Guide Designation B6.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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CDC34 B8.2 gRNA

Plasmid

#90593

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC34

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDC34 (Guide Designation B8.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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SPAG5 G8.2 gRNA

Plasmid

#90901

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPAG5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SPAG5 (Guide Designation G8.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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TUBG1 F8.4 gRNA

Plasmid

#90931

Purpose

3rd generation lentiviral gRNA plasmid targeting human TUBG1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TUBG1 (Guide Designation F8.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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BUB3 A9.1 gRNA

Plasmid

#90556

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BUB3 (Guide Designation A9.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR-ATF7IPi1

Plasmid

#59614

Purpose

Expression of shRNA against human ATF7IP

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

ATF7IP (ATF7IP Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only
pX330-LAMP1

Plasmid

#207787

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pTargetF-FRT

Plasmid

#113637

Purpose

Derivative of pTargetF with gRNA targeting FRT site

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

sgRNA-FRT

Use

CRISPR

Tags

Expression

Mutation

Promoter

pJ23119

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-itis pBE-t

Plasmid

#195342

Purpose

Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V

Depositor

Alexis Komor

Article

Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

Inserts

ecTadA(8e)-SpCas9-NG
gRNA ctctacgcgggtcttgtagt

Use

CRISPR

Tags

Expression

Mutation

SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

Promoter

Lac and ProC

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAcUW51-gEgIL

Plasmid

#11622

Depositor

Pamela Bjorkman

Article

Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

Insert

HSV-1 glycoprotein E and HSV-1 glycoprotein I

Use

Tags

His

Expression

Insect

Mutation

Dicistronic vector containing HSV-1 gE (KOS strai…

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1-shQKI-3373

Plasmid

#115456

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shQKI-3373 (QKI Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMCB320-sgNC.SPA

Plasmid

#169834

Purpose

Expresses a negative control sgRNA

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

safe harbor sgRNA

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 Neo sgTREX1

Plasmid

#127645

Purpose

Knock-out of human TREX1 with NeoR

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

TREX1 sgRNA (TREX1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC34

Plasmid

#62331

Purpose

sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x MS2(wt+f6) binding module
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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Rosa26_gRNA

Plasmid

#196138

Purpose

gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Rosa26 gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-FAM208A ts1

Plasmid

#115871

Purpose

FAM208a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

FAM208a shRNA (C3orf63 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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STK17B gRNA (BRDN0001146764)

Plasmid

#76798

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK17B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK17B (STK17B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJZC33

Plasmid

#62330

Purpose

sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x MS2 binding module
MCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLCKO_LacZ_sgRNA

Plasmid

#74179

Purpose

lentiviral vector expressing sgRNA targeting LacZ

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

LacZ sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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