We narrowed to 7,768 results for: crispr c plasmids

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pJAK184.tetR.PT5-3.dCas9

Plasmid

#232482

Purpose

optimized ahTet inducible dCas9 for insertion into C. difficile genome

Depositor

James Collins

Publication

Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

Insert

Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system

Use

CRISPR

Expression

Bacterial

Promoter

TetR-PT5-3

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV312.3

Plasmid

#119944

Purpose

Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

Depositor

Gerald Schwank

Publication

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pIW447-CRISPR XYL2

Plasmid

#98906

Purpose

K. marxianus CRISPR Plasmid for XYL2 editing

Depositor

Ian Wheeldon

Publication

Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

Inserts

Codon optimized Cas9
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Tags

SV40

Expression

Yeast

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pX459-puro-hMYC

Plasmid

#185376

Purpose

For mammalian expression of guide RNA: TGCTGCCAAGAGGGTCAAGT that targets human MYC

Depositor

Ayaz Najafov

Insert

MYC (MYC Human)

Expression

Mammalian

Mutation

WT

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLV312.3

Plasmid

#119944

Purpose

Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

Depositor

Gerald Schwank

Publication

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

Plasmid

#115136

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Publication

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lentiCRISPR v2-sgMyc_2

Plasmid

#138346

Purpose

Express guide RNA 1 for mouse Myc

Depositor

Yi Zhang

Publication

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

cMyc sgRNA (Myc Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

Plasmid

#115140

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Publication

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

Plasmid

#115138

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Publication

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMLS279

Plasmid

#73729

Purpose

SapTrap FLP-on C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

C-terminal FLP-on

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMLS279

Plasmid

#73729

Purpose

SapTrap FLP-on C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

C-terminal FLP-on

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKLV2-U6gRNA5(sgCXCR1-2)-PGKpuro2ABFP-W

Plasmid

#252916

Purpose

Expression vector for gRNA with Puro and tagBFP selection markers

Depositor

Sok Ching Cheong

Insert

CXCR1 (CXCR1 Human)

Use

CRISPR

Promoter

human U6 promoter, mouse PGK promoter

Available since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(sgCXCR1-1)-PGKpuro2ABFP-W

Plasmid

#252915

Purpose

Expression vector for gRNA with Puro and tagBFP selection markers

Depositor

Sok Ching Cheong

Insert

CXCR1 (CXCR1 Human)

Use

CRISPR

Promoter

human U6 promoter, mouse PGK promoter

Available since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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Plas-CRISPR_reporter

Plasmid

#157658

Purpose

CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

Depositor

Frank Albert

Publication

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

dCAS9-VP64, mCherry, KanMX

Use

Insert storage (replicative in e. coli)

Mutation

N28D in mCherry- please see depositor comments be…

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

Plasmid

#115140

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Publication

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

Plasmid

#115138

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Publication

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28b-SpRY-His

Plasmid

#179320

Purpose

Plasmid for bacterial expression and purification of SpRY-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Publication

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpRY

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

Plasmid

#115137

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Publication

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2.1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

Plasmid

#115139

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Publication

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3.1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRISPR-Cas9/CD4-TK2

Plasmid

#104397

Purpose

The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.

Depositor

Jeronimo Blanco

Publication

Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39

Insert

TK2 (TK2 Human)

Expression

Mammalian

Promoter

U6

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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