Addgene

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pGD250

Plasmid

#132371

Purpose

contains the sequence of mClover3 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-mClover3

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGD251

Plasmid

#132372

Purpose

contains the sequence of mCerulean3 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-mCerulean3

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pRD134

Plasmid

#132378

Purpose

contains the sequence of mScarlet for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mScarlet

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGD165

Plasmid

#132370

Purpose

contains the sequence of eGFP for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-eGFP

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pRD53

Plasmid

#132377

Purpose

contains the sequence of mCherry for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mCherry

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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PS-mOrange2-C1

Plasmid

#54848

Purpose

Localization: C1 Cloning Vector, Excitation: 548 / 634, Emission: 565 / 662

Depositor

Michael Davidson, Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Type

Empty backbone

Use

Tags

PS-mOrange2

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pGD255

Plasmid

#132376

Purpose

contains the sequence of Venus for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-Venus

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pmRuby2-IRES-Clover

Plasmid

#171059

Purpose

Expresses mRuby2 and mClover2 in the cytoplasm as negative control for FRET.

Depositor

Ke Xu

Article

Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi

Insert

IRES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGD253

Plasmid

#132374

Purpose

contains the sequence of mRuby2 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mRuby2

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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LV-Camk2-ChroMEd15-mTFP

Plasmid

#184240

Purpose

channelrhodopsin ChroME fused to mTFP

Depositor

Yiyang Gong

Article

Beck et al Biophys J. 2022 Mar 21. pii: S0006-3495(

Insert

ChroMEd15-mTFP

Use

Lentiviral

Tags

mTFP1

Expression

Mammalian

Mutation

deleted ChroME amino acids 317-331

Promoter

Camk2

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGD252

Plasmid

#132373

Purpose

contains the sequence of TSapphire for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-TSapphire

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGD254

Plasmid

#132375

Purpose

contains the sequence of mOrange for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mOrange

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pRD141

Plasmid

#132379

Purpose

contains the sequence of mKate2 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mKate2

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGD318

Plasmid

#129735

Purpose

Contains a myristoylation sequence in the pGGC000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

Myristoylation sequence (MGRRKRKPK-)

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLII12Pglu-700uDelta6

Plasmid

#17866

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Use

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pKeratin-LSSmOrange

Plasmid

#37138

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human cytokeratin 18

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-rsTagRFP

Plasmid

#31927

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Use

Tags

His

Expression

Bacterial

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

araB

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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prsTagRFP-C1

Plasmid

#31926

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Use

Tags

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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GLUT1-eGFP/pcDNA-DEST47

Plasmid

#18729

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

pcDNA-DEST47 /hGLUT1-eGFP

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pBad-mAmetrine1.1

Plasmid

#18084

Purpose

Bacterial expression of mAmetrine (version 1.1) fluorescent protein

Depositor

Robert Campbell

Article

Ai et al Nat Methods. 2008 May . 5(5):401-3.

Insert

mAmetrine1.1

Use

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only