We narrowed to 649 results for: pBR322

1. piggybac-EF1-halo-mTBP

   Plasmid

   #197908

   Purpose

   single-particle tracking for mTBP

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   TBP (Tbp Mouse)
   

   Use

   Tags

   Halotag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PBDGTV

   Plasmid

   #100859

   Purpose

   A piggyBac transposon based dual directional gene trap vector, in which, the mutagens are two non-selectable gene-trap cassettes in opposite orientations.

   Depositor

   Yue Huang

   Article

   Liu et al Nucleic Acids Res. 2017 Sep 28. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AB65_pCAG.Cas9-D10A.rBGpA

   Plasmid

   #199256

   Purpose

   Expression construct encoding the SpCas9-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   SpCas9.D10A nickase
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRnc-gGFP161

   Plasmid

   #189536

   Purpose

   Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

   Depositor

   Marc Ostermeier

   Article

   Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

   Inserts

   RNase III
   gUTR-161-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   J23119 and pBAD

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AV11_pDonor.EP.S1

   Plasmid

   #100296

   Purpose

   AAVS1-targeting expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   PuroR.T2A.EGFP expression unit
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJin-264

   Plasmid

   #125717

   Purpose

   ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19

   Depositor

   Bryan Dickinson

   Article

   Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

   Insert

   PT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZH051

   Plasmid

   #47311

   Purpose

   Expresses Tsr-Venus-Ubiquitin-CI

   Depositor

   Jie Xiao

   Article

   Hensel et al Nat Struct Mol Biol. 2012 Aug;19(8):797-

   Insert

   wild type immunity region of lambda phage with tsr-venus-ub-cI
   

   Use

   Tags

   CI

   Expression

   Bacterial

   Mutation

   Promoter

   PR; PRM

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

8. hGR-Dx

   Plasmid

   #133408

   Purpose

   overexpression of a mutant human glucocorticoid receptor in mammalian cells

   Depositor

   Andrew Cato

   Article

   Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

   Insert

   NR3C1 (NR3C1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N454D, A458T, R460D, D462C

   Promoter

   Available since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJH579

   Plasmid

   #172163

   Purpose

   pylBCD variant in pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Para (pylBCD JM10.1); AraC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Sumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJin-239

    Plasmid

    #125716

    Purpose

    ABA-inducible split T7 gRNA for GFP vector using RNAPN N-29-1

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    pT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS1

    Plasmid

    #55749

    Purpose

    Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    KanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. DPY-pNCS

    Plasmid

    #17365

    Depositor

    Stephen Goff

    Article

    Yuan et al J Virol. 2000 Aug . 74(16):7250-60.

    Insert

    MMLV proviral DNA
    

    Use

    Tags

    Expression

    Mutation

    deletion that removes 5 amino acids (DPPPY) from …

    Promoter

    Available since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pAGH51

    Plasmid

    #107255

    Purpose

    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.

    Depositor

    Erin O'Shea

    Article

    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

    Insert

    HU-FRB
    

    Use

    Synthetic Biology

    Tags

    FRB

    Expression

    Mutation

    Promoter

    native HU promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAWV

    Plasmid

    #62710

    Purpose

    Donor vector for amiR-eGFP knock-in via landing pad

    Depositor

    Masato Ohtsuka

    Article

    Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

    Insert

    tdTomato/amiR-eGFP123/amiR-eGFP419/FLPe
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Bxb1-GT_TRE-mScarlet

    Plasmid

    #229796

    Purpose

    Bxb1-GT donor plasmid for doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mScarlet
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bxb1-GA_TRE-jRCaMP1b

    Plasmid

    #229798

    Purpose

    Bxb1-GA donor plasmid for doxycycline-inducible expression of jRCaMP1b calcium reporter.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    jRCaMP1b
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. dSp-Rta Full (1-190)

    Plasmid

    #99662

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    truncated RTA activation domain (1-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYG205

    Plasmid

    #200836

    Purpose

    Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives

    Depositor

    Boris Görke

    Article

    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.

    Insert

    rapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P-BAD

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRnc2-gGFP

    Plasmid

    #189563

    Purpose

    Expresses E. Coli RNase III under control of pBAD promoter with native 5' untranslated region and constitutively expresses gUTR-129-GFP

    Depositor

    Marc Ostermeier

    Article

    Weeks et al Mol Biol Evol. 2023 Feb 27:msad047. doi:

    Inserts

    RNase III
    gUTR-129-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Maintains 5' UTR of rnc

    Promoter

    J23119 and pBAD

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRnc-gGFP129

    Plasmid

    #189537

    Purpose

    Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-129-GFP

    Depositor

    Marc Ostermeier

    Article

    Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

    Inserts

    RNase III
    gUTR-129-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    J23119 and pBAD

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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