We narrowed to 11,549 results for: ANG

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pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98976

Purpose

Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(N863A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98972

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1943

Plasmid

#82565

Purpose

High copy number plasmid containing Ple266 (S100B MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple266

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCSDest2-SpCas9-NLS-3XHA-NLS

Plasmid

#69220

Purpose

Expresses wild type SpCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Clover-MyosinIIA-C-18

Plasmid

#56372

Purpose

Localization: Cytoskeleton, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MyosinIIA

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pK218TK620T

Plasmid

#1192

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

Hsp104 (HSP104 Budding Yeast)

Expression

Yeast

Mutation

changed lysine 218 to threonine and lysine 620 to…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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TB205 △trpC

Bacterial Strain

#230038

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for tryptophan

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △trpC

Bacterial Strain

#230037

Purpose

Fluorescently labelled (GFP) E. coli, auxotrophic for tryptophan

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB205 △proC

Bacterial Strain

#230036

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for proline

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △proC

Bacterial Strain

#230035

Purpose

Fluorescently labelled (GFP) E. coli, auxotrophic for proline

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
PECAM1-mRuby3-T2A-secNluc

Plasmid

#235607

Purpose

Donor plasmid for knock-in of mRuby3-T2A-secNluc into the human PECAM1 locus

Depositor

Kai Wang

Article

Zhao et al Angiogenesis. 2025 Jan 3;28(1):10. doi:

Insert

PECAM1 homologous recombination arms with PuroR drug selection cassette

Use

CRISPR

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgNCKAP1-1

Plasmid

#210133

Purpose

knock out NCKAP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Nck-associated protein 1 (NCKAP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMBEC8

Plasmid

#187599

Purpose

Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

nCas9-BEC-UGI; cas6f, gfp

Expression

Bacterial

Promoter

trc

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-136

Plasmid

#118522

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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PX386 Lactobacillus farciminis Cas9

Plasmid

#68323

Purpose

Lactobacillus farciminis Cas9

Depositor

Feng Zhang

Insert

LfCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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PX382 Staphylococcus pseudintermedius Cas9

Plasmid

#68319

Purpose

Staphylococcus pseudintermedius Cas9

Depositor

Feng Zhang

Insert

SpsCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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PX393 Gluconacetobacter diazotrophicus Cas9

Plasmid

#68330

Purpose

Gluconacetobacter diazotrophicus Cas9

Depositor

Feng Zhang

Insert

GdCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Clover-ZO1-C-14

Plasmid

#56375

Purpose

Localization: Tight Junctions, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

ZO1 (TJP1 Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
tdTurboRFP-LaminB1-10

Plasmid

#58060

Purpose

Localization: Nuclear Envelope, Excitation: 553, Emission: 576

Depositor

Michael Davidson

Insert

LaminB1 (LMNB1 Human)

Tags

tdTurboRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits