We narrowed to 32,136 results for: Eng

1. PX459-gR2A_Hinge

   Plasmid

   #124811

   Purpose

   Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Cas9 – gRNA_R2A_hinge
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Bacterial and Mammalian

   Mutation

   R166H in PuroR (please see depositors comment bel…

   Promoter

   pUC

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHybr_r2a>mG1-srt-his

   Plasmid

   #124802

   Purpose

   HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgG1
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   -

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE-ERBB2-L755_T759delLRENT

   Plasmid

   #116339

   Purpose

   Lentiviral expression of ERBB2 L755_T759delLRENT

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   L755_T759delLRENT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE-KRAS-A146V

   Plasmid

   #116422

   Purpose

   Lentiviral expression of KRAS A146V

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   A146V

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHAGE-ERBB2-G776delinsVC

   Plasmid

   #116331

   Purpose

   Lentiviral expression of ERBB2 G776delinsVC

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G776delinsVC

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE-ERBB2-R678Q

   Plasmid

   #116347

   Purpose

   Lentiviral expression of ERBB2 R678Q

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   R678Q

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPB-AMBRA1-501*-3xFLAG

   Plasmid

   #174160

   Purpose

   PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Missing aa 501-1208

   Promoter

   EF1A

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-AMBRA1-217*-3xFLAG

   Plasmid

   #174161

   Purpose

   PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Missing aa 217-1208

   Promoter

   EF1A

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHAGE-ERBB2-V777_G778insGSP

   Plasmid

   #116357

   Purpose

   Lentiviral expression of ERBB2 V777_G778insGSP

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   P780_Y781insGSP

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-ERBB2-L841V

    Plasmid

    #116341

    Purpose

    Lentiviral expression of ERBB2 L841V

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L841V

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHAGE-ERBB2-T862A

    Plasmid

    #116354

    Purpose

    Lentiviral expression of ERBB2 T862A

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T862A

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE-MAP2K4-G294E

    Plasmid

    #116430

    Purpose

    Lentiviral expression of MAP2K4 G294E

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MAP2K4 (MAP2K4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G294E

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE-PTPN11-G60R

    Plasmid

    #116643

    Purpose

    Lentiviral expression of PTPN11 G60R

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G60R

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE-PTPN11-E139D

    Plasmid

    #116640

    Purpose

    Lentiviral expression of PTPN11 E139D

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E139D

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHybr_r2a>mG3-srt-his

    Plasmid

    #124805

    Purpose

    HDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG3
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-PTPN11-T73I

    Plasmid

    #116647

    Purpose

    Lentiviral expression of PTPN11 T73I

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T73I

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-Cry2-mCherry- YAP-N-IDR

    Plasmid

    #166446

    Purpose

    Expresses fusion of CRY2PHR with mCherry and YAP-N-IDR

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    Cry2, mCherry, YAP-N-IDR(1-164) (YAP1, CRY2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHybr_r2a>Fab-srt-his

    Plasmid

    #124810

    Purpose

    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    rat IgG2a Hinge-G4S-Sortag-Histag
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial

    Mutation

    Promoter

    pUC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-PTPN11-N58K

    Plasmid

    #116645

    Purpose

    Lentiviral expression of PTPN11 N58K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    N58K

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-PTPN11-E69K

    Plasmid

    #116641

    Purpose

    Lentiviral expression of PTPN11 E69K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E69K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge