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pMK33/intein-Cas9_S219-3XFLAG

Plasmid

#133782

Purpose

Expresses human codon-optimized intein-Cas9

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Insert

Human codon-optimized intein-Cas9

Expression

Insect

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pExp_TRE:TAL14

Plasmid

#51794

Purpose

Inducible expression of TAL14 as input repressor

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

TAL14

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-EF-gAAVS1

Plasmid

#112262

Purpose

Express g-AAVS1

Depositor

Zhili Rong

Article

Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

Insert

g-AAVS1

Expression

Mammalian

Promoter

U6

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pExp_TRE:TAL21

Plasmid

#51795

Purpose

Inducible expression of TAL21 as input repressor

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

TAL21

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAJF1139

Plasmid

#252908

Purpose

For constitutive expression of GFP-NBALFA. Contains oriM (mycobacterial origin of replication) and kanamycin-resistance cassette.

Depositor

Michael Glickman

Article

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

GFP-NBALFA fusion

Tags

GFP

Expression

Bacterial

Promoter

PftsZ

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAJF1141

Plasmid

#252909

Purpose

For constitutive expression of GFP-NBALFA. Contains oriM (mycobacterial origin of replication) and kanamycin-resistance cassette.

Depositor

Michael Glickman

Article

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

GFP-NBALFA fusion

Tags

GFP

Expression

Bacterial

Promoter

PgroL

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAJF1120

Plasmid

#252903

Purpose

Tet-inducible GFP-NBALFA fusion plasmid. Contains oriM and kanamycin-resistance cassette.

Depositor

Michael Glickman

Article

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

GFP-NBALFA fusion

Tags

GFP

Expression

Bacterial

Promoter

Tet-inducible promoter

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pJPC668

Plasmid

#253814

Purpose

Fluorescent reporter AmCyan

Depositor

Daniel Voytas

Article

Tibebu et al Front. Plant Sci. 17:1794888.

Insert

AmCyan

Use

Golden gate subcloning vector

Promoter

none

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pET15b_RspCas13d-6xHIS

Plasmid

#251218

Purpose

Bacterial overexpression and recombinant protein purification of RspCas13d.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

RspCas13d

Tags

HIS

Expression

Bacterial

Promoter

lac

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pET15b_LshCas13a-6xHIS

Plasmid

#251215

Purpose

Bacterial overexpression and recombinant protein purification of LshCas13a.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

LshCas13a

Tags

HIS

Expression

Bacterial

Promoter

lac

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pET15b_LwaCas13a-6xHIS

Plasmid

#251214

Purpose

Bacterial overexpression and recombinant protein purification of LwaCas13a.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

LwaCas13a

Tags

HIS

Expression

Bacterial

Promoter

lac

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pET15b_LbuCas13a-6xHIS

Plasmid

#251213

Purpose

Bacterial overexpression and recombinant protein purification of LbuCas13a.

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

LbuCas13a

Tags

HIS

Expression

Bacterial

Promoter

lac

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pEF-1a_EGFP_IRES_BSD_WPRE

Plasmid

#251221

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven EGFP expressing cells

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-XRN2_sgRNA_1

Plasmid

#242895

Purpose

PiggyBac cargo vector with XRN2 sgRNA 1 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Murvin et al bioRxiv 2025.07.16.665154

Insert

XRN2 (Xrn2 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-XRN2_sgRNA_2

Plasmid

#242896

Purpose

PiggyBac cargo vector with XRN2 sgRNA 2 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Murvin et al bioRxiv 2025.07.16.665154

Insert

XRN2 (Xrn2 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBIG1A-eIF3klef

Plasmid

#240037

Purpose

Human eIF3k, eIF3l, eIF3e and eIF3e subunits for further cloning or expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3k,l, e, f

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBIG1B-eIF3hdm

Plasmid

#240038

Purpose

Human eIF3h, eIF3d and eIF3m subunits for further cloning or expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3h, d, m

Tags

eIF3d: HA

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTW334f2

Plasmid

#237316

Purpose

expresses A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 G. max Raf1

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 G. max Raf1

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTA7001-RWT4Ca

Plasmid

#237529

Purpose

Expression in Nicotiana benthamiana

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Plant

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pTA7001-RWT4Cs

Plasmid

#237530

Purpose

Expression in Nicotiana benthamiana

Depositor

Gitta Coaker

Article

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Cs

Expression

Plant

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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