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pKFSS1_Psyn-sgRNA500

Plasmid

#149557

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn and pSyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pB24

Plasmid

#61153

Purpose

Expression of Hnt1

Depositor

Charles Brenner

Article

Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

Insert

Hnt1 (HNT1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pBSV2_Psyn-mCherryBb

Plasmid

#149636

Purpose

Psyn-mCherry promoter strength reporter for use in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

Psyn-mCherryBb

Tags

mCherry

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP19-L3S1P51_T

Plasmid

#127037

Purpose

Basic Part T- Terminator; L3S1P51; synthetic terminator

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S1P51_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pbad_P

Plasmid

#127003

Purpose

Basic Part P- Promoter (non-constitutive); Pbad (I13453), arabinose induced; controlled by AraC.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pbad

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-T7pro_P

Plasmid

#127002

Purpose

Basic Part P- Promoter (non-constitutive); T7 promoter; recognized by T7 polymerase

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part T7 promoter_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Streptag_N

Plasmid

#127034

Purpose

Basic Part N- N-terminus; Strep-tag II

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part StrepII_tag_N

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Smad4 R100T

Plasmid

#80891

Purpose

mammalian expression of Smad4 R100T

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

Smad4 (SMAD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Pancreatic cancer mutation R100T

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pJUMP47ts-2A(sfGFP)

Plasmid

#126992

Purpose

Level 2 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-L3S1P51_CT

Plasmid

#127047

Purpose

Basic Part CT- C-terminus + Terminator; L3S1P51. C-termini STOP + terminator (synthetic)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S1P51_CT

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-mCherry_O

Plasmid

#127025

Purpose

Basic Part O- ORF; mCherry; fluorescent protein

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part mCherry_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23107_P

Plasmid

#127000

Purpose

Basic Part P- Promoter (constitutive); J23107.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23107_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-J23100_P

Plasmid

#126996

Purpose

Basic Part P- Promoter (constitutive); J23100

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23100_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pESC/CURT_fluoB

Plasmid

#117997

Purpose

Expression of CURT_fluoB controlled by the Gal1 promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CURT_fluoB

Use

Yeast/bacteria binary vector

Expression

Yeast

Promoter

Gal1

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO CloverCP

Plasmid

#68444

Purpose

TOPO construct expressing CloverCP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

CloverCP

Use

Gmap

Tags

"CP" degradation domain

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO mT2

Plasmid

#68491

Purpose

TOPO construct expressing mT2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mTurquoise2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Smad4 P130S

Plasmid

#80892

Purpose

mammalian expression of Smad4 P130S

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

SMAD4 (SMAD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

colorectal cancer mutation P130S; additional D2E

Promoter

CMV

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nup88(1-550)

Plasmid

#64282

Purpose

Mammalian expression of N-terminal domain of Nup88

Depositor

Birthe Fahrenkrog

Article

Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

Insert

Nup88 (NUP88 Human)

Tags

his and myc

Expression

Mammalian

Mutation

N-terminal domain (1-550)

Promoter

CMV

Available Since

July 14, 2015

Availability

Academic Institutions and Nonprofits only
pNA-Z

Plasmid

#238995

Purpose

Node A carrying sgRNA-2 that can be used for the assembly of pEND-Z11 or pEND-ZY

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

sgRNA-2

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-11Y

Plasmid

#238999

Purpose

Final circuit with -Ara: OFF, +Ara: OFF behaviour

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

Circuit with no sfGFP expression (-Ara: OFF, +Ara: OFF)

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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