We narrowed to 13,169 results for: Mpl;

1. pCAG-TWIN-STREP-FLAG-FLCN

   Plasmid

   #164981

   Purpose

   FLCN expressing vector for mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

   Insert

   FLCN (FLCN Human)
   

   Use

   Tags

   2xSTREP FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-Jun4A-Myc

   Plasmid

   #47444

   Purpose

   mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)

   Depositor

   Axel Behrens

   Article

   Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

   Insert

   Jun 4A mutant (Jun Mouse)
   

   Use

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   four N-terminal residues phosphorylated by JNK, s…

   Promoter

   CMV

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. APEX2-6xHp

   Plasmid

   #214411

   Purpose

   Lipid droplet localization of APEX2-6xHp for electron microscopy

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   6x Spastin aa 43-92 (APEX2 Human)
   

   Use

   Tags

   APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV[Exp]-Puro-EF-1α>{H2B-RFP}

   Plasmid

   #201121

   Purpose

   RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

   Depositor

   Ori Bar-Nur

   Article

   Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

   Insert

   H2B-RFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MBP-FUS_LC_12E

   Plasmid

   #98654

   Purpose

   Expresses MBP-tagged phosphomimetic FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   12 S/T to E

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.3−3xFLAG−AGO2(S387A)

   Plasmid

   #220462

   Purpose

   Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Serine 387 to alanine

   Promoter

   CMV

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag Leucyl tRNA synthetase pRK5

   Plasmid

   #46341

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   leucyl tRNA synthetase (LARS1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

8. myc-Atg14 (396-492)

   Plasmid

   #86755

   Purpose

   N-terminal myc-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

   Insert

   atg14 (ATG14 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   aa 396-492

   Promoter

   CMV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. myc-Atg14 (181-413)

   Plasmid

   #86754

   Purpose

   N-terminal myc-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

   Insert

   atg14 (ATG14 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   aa 181-413

   Promoter

   CMV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. myc-Atg14 (201-492)

    Plasmid

    #86751

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 201-492

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP scFv [N86/8]

    Plasmid

    #199429

    Purpose

    Mammalian expression of GFP scFv. Derived from hybridoma N86/8.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targetingGFP (Aequorea victoria)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEBTetBl-CLIP-Cep128

    Plasmid

    #136872

    Purpose

    Mammalian expression of the centrosomal protein Cep128 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep128 (CEP128 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192789

    Purpose

    aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody minimizer
    

    Use

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBTet-SNAP-Cep97

    Plasmid

    #136800

    Purpose

    Mammalian expression of the centrosomal protein Cep97 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep97 (CEP97 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. P-rTetO-mCherry-T2A-RPL22-1xV5

    Plasmid

    #170325

    Purpose

    Tetracycline-inducible expression of V5 and mCherry tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5

    Plasmid

    #170328

    Purpose

    Double-floxed inverse Orientation V5 and mCherry tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. shBIRC5 # 2

    Plasmid

    #42554

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shBIRC5 (BIRC5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pOPINO_H11

    Plasmid

    #184277

    Purpose

    Expresses nanobody H11 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11
    

    Use

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAVS1-EF1a-mTagRFP-T-CAAX

    Plasmid

    #167973

    Purpose

    AAVS1 targeting repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    AAVS1 (PPP1R12C, AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mTagRFP-T-CAAX

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. shBIRC5 # 1

    Plasmid

    #42553

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shBIRC5 (BIRC5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

21. Rab8-T22N(Flag) in pcDNA3.1neo

    Plasmid

    #46784

    Purpose

    expression of T22N Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A T22N (RAB8A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T22N dominant negative

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid

    #84244

    Purpose

    Expresses GA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sa dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

    Plasmid

    #115787

    Purpose

    Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    FZD4 (FZD4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEBTet-SNAP-ALMS1

    Plasmid

    #136828

    Purpose

    Mammalian expression of the centrosomal protein ALMS1 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-ALMS1 (ALMS1 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pIRES:mTREK-1(G137I)

    Plasmid

    #133274

    Purpose

    Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G137I

    Promoter

    CMVd2

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pmKO-imp α

    Plasmid

    #200082

    Purpose

    Expresses mKO-tagged human importinα in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

    Insert

    importin α (KPNA2 Human)
    

    Use

    Tags

    mKO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFL His-SDS22 / PP1gamma

    Plasmid

    #113511

    Purpose

    Expression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Inserts

    SDS22 (PPP1R7 Human)
    PP1gamma (PPP1CC Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

    Plasmid

    #63139

    Purpose

    Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

    Depositor

    Titia Sixma

    Article

    Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

    Inserts

    Ring1b (Ring1 Mouse)
    Bmi1 (Bmi1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-109 and Residues 1-159

    Promoter

    tac

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA3.1/NDUFV1/myc-His

    Plasmid

    #127490

    Purpose

    Expresses myc-tagged NDUFV1 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFV1 (NDUFV1 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

    Plasmid

    #74261

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.Y591X truncation

    Promoter

    CMV

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pcDNA5-FRT-TO-FH-Nsp13_GC3opt

    Plasmid

    #157715

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. Cav3P104L-YFP

    Plasmid

    #68404

    Purpose

    Expression of fluorescently tagged Caveolin3 point mutation in mammalian cells

    Depositor

    Rob Parton

    Article

    Pol et al Mol Biol Cell. 2005 Apr;16(4):2091-105.

    Insert

    Cav3 (Cav3 Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Proline at position 104 changed to a Leucine

    Promoter

    CMV

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pAd-Track HA PGC-1 alpha

    Plasmid

    #14427

    Depositor

    Pere Puigserver

    Article

    Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Adenoviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Last four amino acids of PGC-1a removed to make t…

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

34. pEN_TT 3xFLAG-MAVS

    Plasmid

    #158628

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged MAVS

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Gateway entry vector

    Tags

    3XFLAG

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-mitoX4-mCerulean

    Plasmid

    #185807

    Purpose

    Mitochondrial targetted (COX8) mCerulean

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    mito-mCerulean
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. eGFP Depdc5 pLJM1

    Plasmid

    #46380

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Differs from Depdc5 isoform 1 by 10 amino acids …

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pCI-Myc3-HPS4

    Plasmid

    #133931

    Purpose

    Expresses 3xMyc-HPS4 construct in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

    Insert

    HPS4 (HPS4 Human)
    

    Use

    Tags

    3xMyc tag

    Expression

    Mammalian

    Mutation

    Glutamic acid 229 to Glycine; Valine 552 to Methi…

    Promoter

    CMV I.E.

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSICO-CPSF6-358-eGFP

    Plasmid

    #110693

    Purpose

    Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP

    Depositor

    Zandrea Ambrose

    Article

    Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

    Insert

    CPSF6-eGFP (CPSF6 )
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Truncation of CPSF6 isoform 2 at amino acid 358

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLJM1 FLCN (R164A) FLAG GFP

    Plasmid

    #164980

    Purpose

    Lentiviral vector for FLCN expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FLCN (FLCN Human)
    

    Use

    Lentiviral

    Tags

    1xFLAG-GFP

    Expression

    Mammalian

    Mutation

    R164A

    Promoter

    UBC

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEBTet-SNAP-Cep78

    Plasmid

    #136827

    Purpose

    Mammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep78 (CEP78 Human, Synthetic)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge