We narrowed to 4,193 results for: Pet2

1. pLyGo-Ec-6

   Plasmid

   #163132

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a-His6-SUMO-VAMP8

   Plasmid

   #135553

   Purpose

   Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP8
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p28atDarpp

   Plasmid

   #108318

   Purpose

   Expresses tDarpp in E.coli

   Depositor

   Jamil Momand

   Article

   Momand et al FEBS Open Bio. 2017 Aug 29;7(9):1328-133

   Insert

   tDarpp (PPP1R1B Human)
   

   Use

   Tags

   6 Histadine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-ZZ(1700-1751)

   Plasmid

   #99334

   Purpose

   Bacterial expression of mouse CBP ZZ domain

   Depositor

   Peter Wright

   Article

   Legge et al J Mol Biol. 2004 Oct 29;343(4):1081-93.

   Insert

   CBP ZZ domain (Crebbp Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZ004 (LseC2c2 locus)

   Plasmid

   #70170

   Purpose

   Expresses LseC2c2 locus with one spacer in array

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   LseC2c2 locus with one spacer in array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

6. HOXA9 (murine) FLAG pET

   Plasmid

   #8494

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HOXA9 (Hoxa9 Mouse)
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   FLAG epitope tag replaces T7 tag in the pET back…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

7. HOXB13 (human) HIS-tag pET

   Plasmid

   #8543

   Depositor

   Corey Largman

   Article

   Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   makes protein by Western and by transcription/tra…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. OphA-delta C6

   Plasmid

   #127679

   Purpose

   Bacterial expression of His-tagged OphA-delta C6

   Depositor

   James Naismith

   Article

   Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

   Insert

   OphA-delta C6
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   delta C6

   Promoter

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. L3MBTL3 (3UT1)

   Plasmid

   #36901

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   L3MBTL3 (L3MBTL3 Human)
   

   Use

   Tags

   His tag and Streptavidin-Binding Peptide (SBP)-Ta…

   Expression

   Bacterial

   Mutation

   contains amino acid residues 228-551 only

   Promoter

   Available since

   June 21, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

10. MolBC

    Plasmid

    #92210

    Purpose

    Expression of H. influenzae MolBC (ABC Transporter) in E. coli

    Depositor

    Douglas Rees

    Article

    Pinkett et al Science. 2007 Jan 19;315(5810):373-7. Ep

    Inserts

    MolC (HI1471 )
    MolB (HI1470 )
    

    Use

    Tags

    8x Histidine Tag and Enterokinase Cleavage Site b…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human USP30 (64-502, construct 13i, C77A, MG-31-28)

    Plasmid

    #110747

    Purpose

    Bacterial expression of human USP30 (construct 13i)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    C77A, F348D, M350S, I353E + insertion deletion: 6…

    Promoter

    T7

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-NEMOL329P

    Plasmid

    #11969

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMOL329P (IKBKG Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Leucine329 to Proline

    Promoter

    Available since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

13. HOXD13 (chicken) HIS-tag pET

    Plasmid

    #8555

    Depositor

    Corey Largman

    Article

    Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206

    Insert

    HOXD13 (HOXD13 )
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    contains 10 nt 5' to ATG start; makes prote…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. Dom34(265-386)

    Plasmid

    #19102

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

15. Dom34(265-377)

    Plasmid

    #19103

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

16. Human Mevalonate diphosphate decarboxylase (MDD)

    Plasmid

    #52431

    Purpose

    bacterial expression of human Mevalonate diphosphate decarboxylase (MDD)

    Depositor

    Henry Miziorko

    Article

    Voynova et al Arch Biochem Biophys. 2008 Dec 1;480(1):

    Insert

    mevalonate diphosphate decarboxylase (MVD Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

17. NLuc-His10

    Plasmid

    #234567

    Purpose

    Bacterially expressed NLuc for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    Nano luciferase
    

    Use

    Tags

    T7 gene 10 leader peptide and Thrombin site-10xHi…

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    T7

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. NP-11-63

    Plasmid

    #234358

    Purpose

    Expressing and purifying R.rubrum rubisco using a his-sumo tag

    Depositor

    David Savage

    Article

    Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

    Insert

    R.rubrum rubisco
    

    Use

    Synthetic Biology

    Tags

    N-term SUMO tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Kif3A-EGFP-SNAP

    Plasmid

    #229852

    Purpose

    Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

    Depositor

    William Hancock

    Insert

    Kif3A (Kif3a Mouse)
    

    Use

    Tags

    6xHis, EGFP, and SNAP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. MZB215

    Plasmid

    #221767

    Purpose

    Bacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tag

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    CPD-10xHis

    Expression

    Bacterial

    Mutation

    Q67L

    Promoter

    T7 Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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