We narrowed to 11,282 results for: Vars

1. pFA6a-FLAG-APEX2-NES-TRP1

   Plasmid

   #229228

   Purpose

   Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

   Depositor

   Fulvio Reggiori

   Article

   Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS puro human RLIM N581K

   Plasmid

   #232242

   Purpose

   Mammalian expression of Human RLIM N581K

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   Human RLIM N581K (RLIM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N581K

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLANT-2b

   Plasmid

   #231487

   Purpose

   Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

   Depositor

   Venkat Gopalan

   Inserts

   SUMO^NIT (SMT3 Budding Yeast)
   FraB
   

   Use

   Tags

   His6 tag and His6-SUMO^NIT

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

   Promoter

   T7 promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1 human RLIM N581K

   Plasmid

   #232241

   Purpose

   Protein expression of GST-human RLIM N581K

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   GST-human RLIM N581K (RLIM Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N581K

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-shRNA2-zsGreen-PGK-puro_HSALNG0104472-163

   Plasmid

   #197902

   Purpose

   For the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.

   Depositor

   Bo Wang

   Article

   Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

   Insert

   HSALNG0104472-163 (HSALNG0104472-scramble-sequence)
   

   Use

   Lentiviral

   Tags

   zsGreen1

   Expression

   Mammalian

   Mutation

   15q11.2 deletion

   Promoter

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Cas9-FCPF

   Plasmid

   #220132

   Purpose

   Expresses Cas9-FCPF in E.coli

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   Cas9-FCPF (cas9 S. pyogenes)
   

   Use

   Tags

   His and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-FCPF

   Plasmid

   #220131

   Purpose

   Expresses dCas9-FCPF in mammalian cells

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   dCas9-FCPF (cas9 Synthetic, S. pyogenes)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-dCas9-FCPF

   Plasmid

   #220133

   Purpose

   Expresses dCas9-FCPF in E.coli

   Depositor

   Xinlai Cheng

   Article

   Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

   Insert

   dCas9-FCPF (cas9 Synthetic, S. pyogenes)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   FCPF sequence inserted on C-terminal

   Promoter

   T7

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDUP2

   Plasmid

   #223517

   Purpose

   cogfp copy 1 under control of Ptet, cogfp copy 2 under control of Ptac

   Depositor

   Yolanda Schaerli

   Article

   Ljiljana et al eLife13:RP97216

   Inserts

   CoGFP
   CoGFP (second copy)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   anti-dimerization mutations S129G, 22 C154S, D156…

   Promoter

   Ptac, Ptac and Ptet, and Ptet

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac1-NpSxph

    Plasmid

    #193097

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Nanorana parkeri saxiphilin
    

    Use

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac1-NpSxphI559Y

    Plasmid

    #193099

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Nanorana parkeri saxiphilin I559Y
    

    Use

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Mutation

    I559Y

    Promoter

    polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1-OsSxph

    Plasmid

    #193100

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Oophaga sylvatica saxiphilin
    

    Use

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac1-MaSxph

    Plasmid

    #193101

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Mantella aurantica saxiphilin
    

    Use

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac1-RiSxph

    Plasmid

    #193102

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Ranitomeya imitator saxiphilin
    

    Use

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. RcSxph E540A

    Plasmid

    #194019

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RcSxph E540D

    Plasmid

    #194020

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540D

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RcSxph E540Q

    Plasmid

    #194021

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540Q

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RcSxph Y558A

    Plasmid

    #194022

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RcSxph Y558F

    Plasmid

    #194023

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558F

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RcSxph Y558I

    Plasmid

    #194024

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558I

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge