We narrowed to 4,413 results for: biorxiv

1. Cultivarium MACKEREL Library

   Pooled Library

   #208054

   Purpose

   pGL0_151 [RS421] is a library of broad host range prokaryotic promoters-RBS regulatory sequences formatted as a golden gate assembly Level 0 part.

   Depositor

   Nili Ostrov, Cultivarium Tools, Charlie Gilbert

   Article

   Gilbert et al bioRxiv 2024.02.23.581749

   Use

   Synthetic Biology

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM)

   Pooled Library

   #198050

   Purpose

   By supplying an AAV cap gene of interest in trans, researchers can use this library to investigate the effects of rep mutations on production of a wide range of AAV capsid serotypes

   Depositor

   George Church

   Article

   Jain et al bioRxiv 2023.01.31.526541

   Expression

   Mammalian

   Use

   AAV

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

   Plasmid

   #234453

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-NGR
   

   Use

   AAV; Flp/frt

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234454

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV; Flp/frt

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pαH-S-GSAS-2P-Omicron-BA.5

   Plasmid

   #213828

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pαH-S-GSAS/Omicron-2P-BA5 (S )
   

   Use

   Tags

   HRV 3C cleavage site (before tags) 8X His tag 2X…

   Expression

   Mammalian

   Mutation

   G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG-FLEX.iAChSnFR-Venus-NULL

   Plasmid

   #137960

   Purpose

   Expresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter

   Depositor

   Loren Looger

   Article

   Borden et al bioRxiv 2020

   Insert

   iAChSnFR (non-binding yellow variant)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Y140A binding site mutation

   Promoter

   CAG

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5D HA-PPM1H_M flap

   Plasmid

   #236718

   Purpose

   Expresses PPM1H with PPM1M flap domain sequence

   Depositor

   Suzanne Pfeffer

   Article

   Claire Y. et al bioRxiv, 2025

   Insert

   PPM1H_M flap (PPM1H, PPM1M Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PPM1H flap domain replaced with PPM1M flap domain

   Promoter

   CMV

   Available since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV5D HA-PPM1M_H flap

   Plasmid

   #236719

   Purpose

   Expresses PPM1M with PPM1H flap domain sequence

   Depositor

   Suzanne Pfeffer

   Article

   Claire Y. et al bioRxiv, 2025

   Insert

   PPM1M_H flap (PPM1M, PPM1H Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PPM1M flap domain replaced with PPM1H flap domain

   Promoter

   CMV

   Available since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.hSynap.iAChSnFR-Venus-NULL

   Plasmid

   #137953

   Purpose

   Expresses iAChSnFR (non-binding yellow version) under hSynapsin promoter

   Depositor

   Loren Looger

   Article

   Borden et al bioRxiv 2020

   Insert

   iAChSnFR (yellow version, non-binding variant)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   cpSFGFP replaced with cpSFVenus; Y140A binding si…

   Promoter

   hSynapsin

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRE-MPRA_minPromega Library

    Pooled Library

    #201013

    Purpose

    Massively parallel reporter assay (MPRA) library with minPromega promoter

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Expression

    Mammalian

    Use

    Luciferase

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

11. scQers promoter control #5 (high: EEF1A1 promoter, p028)

    Pooled Library

    #217424

    Purpose

    scQers promoter control #5 (high: EEF1A1 promoter, p028)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

12. TRE-MPRA_minCMV Library

    Pooled Library

    #201012

    Purpose

    Massively parallel reporter assay (MPRA) library with minCMV promoter

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Expression

    Mammalian

    Use

    Luciferase

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

13. scQers promoter control #2 (low: no promoter, p029)

    Pooled Library

    #217421

    Purpose

    scQers promoter control #2 (low: no promoter, p029)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

14. scQers promoter control #3 (intermediate: UBC promoter, p041)

    Pooled Library

    #217422

    Purpose

    scQers promoter control #3 (intermediate: UBC promoter, p041)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

15. scQers promoter control #4 (intermediate: Pgk1 promoter, p042)

    Pooled Library

    #217423

    Purpose

    scQers promoter control #4 (intermediate: Pgk1 promoter, p042)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

16. scQers promoter control #1 (low: minimal promoter, p027)

    Pooled Library

    #217420

    Purpose

    scQers promoter control #1 (low: minimal promoter, p027)

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

17. pre-associated oBC-mBC backbone (p025)

    Pooled Library

    #194097

    Purpose

    pre-associated oBC-mBC backbone (p025), can insert additional reporter constructs

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

18. TRE-MPRA_minTK Library

    Pooled Library

    #201014

    Purpose

    Massively parallel reporter assay (MPRA) library with minTK promoter

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Expression

    Mammalian

    Use

    Luciferase

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

19. pre-associated oBC-minP-GFP-mBC backbone (p043)

    Pooled Library

    #194098

    Purpose

    pre-associated oBC-minP-GFP-mBC backbone (p043), can directly be used to deposit library of regulatory elements

    Depositor

    Jay Shendure

    Article

    Lalanne et al bioRxiv 2022.12.10.519236

    Expression

    Bacterial and Mammalian

    Use

    RNAi

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

20. pαH-S-GSAS-OMICRON.BA.5

    Plasmid

    #213069

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    S-GSAS-Omicron.BA5 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) C terminal on …

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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