We narrowed to 9,206 results for: sgRNA

Showing: 3841 - 3860 of 9206 results

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pCas9-J23109

Plasmid

#113150

Purpose

Plasmid constitutively expressing Cas9 protein

Depositor

Chong Zhang

Publication

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

Cas9

Expression

Bacterial

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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B-HypaSpCas9

Plasmid

#126763

Purpose

Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

B-HypaSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N692A; M694A; Q695A; H698A; amino acids 1005-1013…

Promoter

CBh

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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PINK1 gRNA (BRDN0001145357)

Plasmid

#78036

Purpose

3rd generation lentiviral gRNA plasmid targeting human PINK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PINK1 (PINK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKDC gRNA (BRDN0001149021)

Plasmid

#77864

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKDC

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKDC (PRKDC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MLKL gRNA (BRDN0001148608)

Plasmid

#77539

Purpose

3rd generation lentiviral gRNA plasmid targeting human MLKL

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MLKL (MLKL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRD4 gRNA (BRDN0001147320)

Plasmid

#77344

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK9 gRNA (BRDN0001146478)

Plasmid

#77169

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK9

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK9 (CDK9 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AKT3 gRNA (BRDN0001146868)

Plasmid

#76217

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT3

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT3 (AKT3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STK11 gRNA (BRDN0001146880)

Plasmid

#75912

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK11

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK11 (STK11 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

Plasmid

#188773

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgENPP1_#2-puro

Plasmid

#254675

Purpose

knockout mouse ENPP1

Depositor

Brian Ruffell

Insert

sgRNA with Cas9 and puromycin resistance (Enpp1 Mouse)

Use

CRISPR and Lentiviral

Available since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgENPP1_#1-puro

Plasmid

#254674

Purpose

knockout mouse ENPP1

Depositor

Brian Ruffell

Insert

sgRNA with Cas9 and puromycin resistance (Enpp1 Mouse)

Use

CRISPR and Lentiviral

Available since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO-CreERT2 stuffer v3

Plasmid

#158046

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Publication

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MLKL gRNA (BRDN0001146456)

Plasmid

#77540

Purpose

3rd generation lentiviral gRNA plasmid targeting human MLKL

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MLKL (MLKL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK2 gRNA (BRDN0001147786)

Plasmid

#77192

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK2 (CDK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK9 gRNA (BRDN0001162576)

Plasmid

#77170

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK9

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK9 (CDK9 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EIF2AK4 gRNA (BRDN0001146469)

Plasmid

#75876

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK4

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK4 (EIF2AK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ULK1 gRNA (BRDN0001145044)

Plasmid

#75736

Purpose

3rd generation lentiviral gRNA plasmid targeting human ULK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ULK1 (ULK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ULK1 gRNA (BRDN0001145850)

Plasmid

#75737

Purpose

3rd generation lentiviral gRNA plasmid targeting human ULK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ULK1 (ULK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EGFR gRNA (BRDN0001146570)

Plasmid

#75688

Purpose

3rd generation lentiviral gRNA plasmid targeting human EGFR

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFR (EGFR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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