We narrowed to 73,629 results for: LAS

1. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80065

   Purpose

   Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Zic2a enhancer driving c-fos promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMTB2-Avi-Cerulean-Rpl10a

   Plasmid

   #79880

   Purpose

   Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
   

   Use

   Tags

   Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Beta-actin2

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEN_TGmiR_Gb2-K

   Plasmid

   #25766

   Purpose

   Entry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Gb2 miR-shRNA (Gnb2 Mouse)
   

   Use

   Entry vector

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOTTC1534 pscAAV mU6 shRNA(PKCd) CMV-IE Nuc-EYFP

   Plasmid

   #135562

   Purpose

   An AAV vector expressing shRNA vs rat PKCd and a nuclear EYFP reporter

   Depositor

   Christopher Richie

   Article

   Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

   Inserts

   shRNA (mouse PKCd)
   Nuc-EYFP
   

   Use

   AAV and RNAi

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE and mU6

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBabe_puro_DEST_Flag_TLOC1 (220aa)

   Plasmid

   #45273

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   TLOC1 (SEC62 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

6. 8200 Bicistronic_GFP_ires_hygro

   Plasmid

   #64375

   Purpose

   This a retroviral expression plasmid expressing GFP along with hygro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HTRA3_PDZ_1

   Plasmid

   #103914

   Purpose

   Protein expression and purification of HTRA3 PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   HTRA3 (HTRA3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBabe_puro_DEST_Flag_SLC7A14

   Plasmid

   #45261

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   SLC7A14 (SLC7A14 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79889

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-FKBP-TPD53

    Plasmid

    #172457

    Purpose

    Expression of Tumor Protein D52 like 1 (TPD53/TPD52L1) with N-terminal mCherry-FKBP tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD53 (TPD52L1 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-STAG2-A223Stop

    Plasmid

    #31973

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    human STAG2 cDNA (STAG2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed Alanine 223 to Stop codon

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80061

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Myl7 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Djun - sense

    Plasmid

    #38337

    Depositor

    Norbert Perrimon

    Article

    Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

    Insert

    djun (Jra Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pLenti CMV Hygro DHFR.cHSF1

    Plasmid

    #134738

    Purpose

    Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

    Insert

    DHFR.cHSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    DHFR

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202

    Promoter

    CMV

    Available since

    Oct. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMTB2-RanGAP-Cerulean-Avi

    Plasmid

    #80068

    Purpose

    Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Zebrafish biotagging

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PDZK1_PDZ_1

    Plasmid

    #103927

    Purpose

    Protein expression and purification of PDZK1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SLC9A3R2_PDZ_2

    Plasmid

    #103926

    Purpose

    Protein expression and purification of NHERF2 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    SLC9A3R2 (NHERF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PDZK1_PDZ_4

    Plasmid

    #103945

    Purpose

    Protein expression and purification of PDZK1 PDZ4 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLIK-Venus-TmiR-G13-G12

    Plasmid

    #25742

    Purpose

    Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 and 12 miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FKBP-beta2(616-951)-GFP Double

    Plasmid

    #100729

    Purpose

    To express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform, deletion of clathrin box motif and …

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge