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pFE-dabAB2 D353A

Plasmid

#133006

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

D353A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-R72K

Plasmid

#127683

Purpose

Bacterial expression of His-tagged OphA-delta C6-R72K

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-R72K

Tags

His8

Expression

Bacterial

Mutation

delta C6-R72K

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C18

Plasmid

#127678

Purpose

Bacterial expression of His-tagged OphA-delta C18

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C18

Tags

His8

Expression

Bacterial

Mutation

delta C18

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y63F

Plasmid

#127680

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y63F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y63F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y63F

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y66F

Plasmid

#127681

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y66F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y66F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y66F

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-R72A

Plasmid

#127682

Purpose

Bacterial expression of His-tagged OphA-delta C6-R72A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-R72A

Tags

His8

Expression

Bacterial

Mutation

delta C6-R72A

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y76F

Plasmid

#127684

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y76F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y76F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y76F

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OpHA-delta C6-Y98A

Plasmid

#127685

Purpose

Bacterial expression of His-tagged OpHA-delta C6-Y98A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OpHA-delta C6-Y98A

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y98A

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Y98F

Plasmid

#127686

Purpose

Bacterial expression of His-tagged OphA-delta C6-Y98F

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Y98F

Tags

His8

Expression

Bacterial

Mutation

delta C6-Y98F

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-Q172A

Plasmid

#127687

Purpose

Bacterial expression of His-tagged OphA-delta C6-Q172A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-Q172A

Tags

His8

Expression

Bacterial

Mutation

delta C6-Q172A

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-W400A

Plasmid

#127688

Purpose

Bacterial expression of His-tagged OphA-delta C6-W400A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-W400A

Tags

His8

Expression

Bacterial

Mutation

delta C6-W400A

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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OphA-delta C6-P399A

Plasmid

#127689

Purpose

Bacterial expression of His-tagged OphA-delta C6-P399A

Depositor

James Naismith

Article

Song et al Sci Adv. 2018 Aug 24;4(8):eaat2720. doi:

Insert

OphA-delta C6-P399A

Tags

His8

Expression

Bacterial

Mutation

delta C6-P399A

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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JK305: pMVP/Blast-DEST

Plasmid

#121855

Purpose

pMVP destination vector, empty expression vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JL104: pMVP/Neo-DEST

Plasmid

#121857

Purpose

pMVP destination vector, empty expression vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29[dCas9]-1A(lacZ)

Plasmid

#126979

Purpose

Level 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

Cas9 catalytically dead

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFP88

Plasmid

#83544

Purpose

Used to evaluate the expression output of RPL15A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL15A promoter-yEGFP

Expression

Yeast

Promoter

RPL15A

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP77

Plasmid

#83538

Purpose

Used to evaluate the expression output of RPL8B promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL8B promoter-yEGFP

Expression

Yeast

Promoter

RPL8B

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTIM

Plasmid

#81198

Purpose

pTIM in vitro transcription backbone plasmid with two multiple cloning sites and two Rho-independent transcriptional terminators.

Depositor

Andrés Vázquez-Torres

Article

Tapscott et al Sci Rep. 2018 Jun 21;8(1):9465. doi: 10.

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFPH0

Plasmid

#83552

Purpose

Used to evaluate the expression output of TPS1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TPS1 promoter-yEGFP

Expression

Yeast

Promoter

TPS1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP90

Plasmid

#83547

Purpose

Used to evaluate the expression output of TEF2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF2 promoter-yEGFP

Expression

Yeast

Promoter

TEF2

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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