We narrowed to 9,148 results for: sgRNA

Showing: 3861 - 3880 of 9148 results

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ipUSEPR-sg-Hs-PHGDH-1

Plasmid

#188675

Purpose

sgRNA

Depositor

Lewis Cantley

Insert

sgPHGDH (PHGDH Human)

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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ipUSEPR-sg-Hs-BCL2L1-A1

Plasmid

#188692

Purpose

sgRNA

Depositor

Lewis Cantley

Insert

sgBCL2L1 (BCL2L1 Human)

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

Plasmid

#188765

Purpose

dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-XTEN80-KRAB(Kox1)

Use

Lentiviral

Tags

HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Promoter

SFFV

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

Plasmid

#168291

Purpose

optimized miniCMV (OminiCMV)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

mCherry

Expression

Mammalian

Promoter

miniCMV

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B5

Plasmid

#172846

Purpose

CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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LHZ1493

Plasmid

#222102

Purpose

Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.

Depositor

Yongming Wang

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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ipUSEPR-sg-Hs-PCNA

Plasmid

#188686

Purpose

control sgRNA

Depositor

Lewis Cantley

Insert

sgPCNA (PCNA Human)

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP2849

Plasmid

#160677

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGY037 BSMV-gamma-AmCyan

Plasmid

#234818

Purpose

To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

AmCyan

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pWR63(YUM1)

Plasmid

#203336

Purpose

episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
YUM1-targeting sequence

Use

CRISPR

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001162235)

Plasmid

#80261

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147780)

Plasmid

#80226

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK1 gRNA (BRDN0001147355)

Plasmid

#77787

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK1 (MAPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK1 gRNA (BRDN0001147498)

Plasmid

#77789

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK1 (MAPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK5 gRNA (BRDN0001145540)

Plasmid

#77542

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK5 (CDK5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRD4 gRNA (BRDN0001146786)

Plasmid

#77346

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GSK3B gRNA (BRDN0001145264)

Plasmid

#77281

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSK3B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSK3B (GSK3B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GSK3B gRNA (BRDN0001146390)

Plasmid

#77282

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSK3B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSK3B (GSK3B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIKFYVE gRNA (BRDN0001147303)

Plasmid

#76892

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIKFYVE (PIKFYVE Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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