We narrowed to 4,320 results for: Pet2;

1. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

   Plasmid

   #153440

   Purpose

   E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc. XDocB binding mode A knock-out mutant
   

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Arginine 191 and leucine 195 on XDocB were mutate…

   Promoter

   T7 promoter

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

   Plasmid

   #111398

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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3. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

   Plasmid

   #111399

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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4. M63 pPET28a-TEV-human beta cat domain C(666-781)

   Plasmid

   #17204

   Depositor

   Randall Moon

   Insert

   beta catenin (CTNNB1 Human)
   

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   carboxy-terminal residues 666-781

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

   Plasmid

   #222288

   Purpose

   Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.

   Depositor

   Anne Straube

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Insert

   KIF1C (KIF1C Human)
   

   Tags

   6xHis, GFP, and GST

   Expression

   Bacterial

   Mutation

   stalk region only amino acids 642 to 922

   Promoter

   T7

   Available Since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

   Plasmid

   #180806

   Purpose

   Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)
   

   Expression

   Bacterial

   Mutation

   BTLA (Y243F, Y257F, Y282F)

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

   Plasmid

   #180807

   Purpose

   Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   BTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)
   

   Expression

   Bacterial

   Mutation

   BTLA (Y226F, Y257F, Y282F)

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

   Plasmid

   #180808

   Purpose

   Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   BTLAint (AA190-289, Y226F, Y243F, Y282F)-TwinStrep (BTLA Human)
   

   Expression

   Bacterial

   Mutation

   BTLA (Y226F, Y243F, Y282F)

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y257F)-TwinStrep

   Plasmid

   #180809

   Purpose

   Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y257F)-TwinStrep

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   BTLAint (AA190-289, Y226F, Y243F, Y257F)-TwinStrep (BTLA Human)
   

   Expression

   Bacterial

   Mutation

   BTLA (Y226F, Y243F, Y257F)

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)

    Plasmid

    #153439

    Purpose

    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Tags

    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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