We narrowed to 4,862 results for: Mela

1. pET-DEST42 -SSRP1

   Plasmid

   #170439

   Purpose

   Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

   Depositor

   Joan Conaway

   Article

   Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

   Insert

   ssrp1 (Ssrp Fly)
   

   Use

   Tags

   6xHis and V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

   Plasmid

   #167200

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R D421N
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

   Plasmid

   #167198

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R E294K
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-3xFLAG-Puro PAK5 K478R/479R M173I

   Plasmid

   #167197

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R M173I
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-3xFLAG-Puro PAK5 K478R/479R E144K

   Plasmid

   #167196

   Purpose

   puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant

   Depositor

   Christin Burd

   Article

   LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

   Insert

   PAK5 K478R/479R E144K
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Dscam1_7.27.26 EC10-Fc-His

   Plasmid

   #72189

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.26
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Dscam1_7.27.23 EC10-Fc-His

   Plasmid

   #72187

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.23
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Dscam1_7.27.26 EC10-AP-His

   Plasmid

   #72063

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.26
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXs_FLAG-Sfxn2 (CG6812)

   Plasmid

   #110643

   Purpose

   Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Science. 2018 Nov 16;362(6416). pii: 362

   Insert

   CG6812 (CG6812 Fly)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon-optimized cDNA (no amino acid mutations)

   Promoter

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs_FLAG-Sfxn1-3 (CG11739)

    Plasmid

    #110642

    Purpose

    Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    CG11739 (CG11739 Fly)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Promoter

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. mei-S332 5.6Kb genomic DNA in Casper4

    Plasmid

    #112984

    Purpose

    P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

    Insert

    mei-S332 (Sgo) (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX6P-1 PUM FL

    Plasmid

    #112997

    Purpose

    For protein expression and purification of Drosophila pumilio (amino acids 566-1090)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    pumilio (pum Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. UASp-deltaN cycB

    Plasmid

    #112967

    Purpose

    P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Lee et al Genetics. 2001 Aug;158(4):1545-56.

    Insert

    cycB (CycB Fly)
    

    Use

    P insertion vector

    Tags

    Expression

    Mutation

    N-term deletion of first 46 amino acids, includes…

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. GAL-ARF

    Plasmid

    #89121

    Purpose

    mammalian expression of p14-hARF linked to GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

    Insert

    p14-hARF (CDKN2A Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. exon K-renilla

    Plasmid

    #81007

    Purpose

    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

    Depositor

    Richard Baines

    Article

    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

    Insert

    paralytic (para Fly)
    

    Use

    Tags

    Renilla luciferase

    Expression

    Insect

    Mutation

    spanning exon 24 to exon 26, termination codon in…

    Promoter

    P-AC5 (Actin 5C)

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Dscam1_7.27.30 EC10-AP-His

    Plasmid

    #72064

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.30
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Dscam1_7.27.22 EC10-AP-His

    Plasmid

    #72060

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.22
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Dscam1_7.27.23 EC10-AP-His

    Plasmid

    #72061

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.23
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Dscam1_7.27.22 EC10-Fc-His

    Plasmid

    #72186

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.22
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFOSGE_COF3H04

    Plasmid

    #70971

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Taf6 (Taf6 Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only