We narrowed to 4,240 results for: erf

1. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187945

   Purpose

   dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   Synthetic Biology; Piggybac

   Tags

   NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTMB400_ZFcharm_Prnp_DPM

   Plasmid

   #220847

   Purpose

   AAV genome expressing ZFcharm targeting Prnp; double perfect match self-silencing binding site

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm
   

   Use

   AAV

   Tags

   HAtag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTMB399_ZFcharm_Prnp_SPM

   Plasmid

   #220846

   Purpose

   AAV genome expressing ZFcharm targeting Prnp; single perfect match self-silencing binding site

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm
   

   Use

   AAV

   Tags

   HAtag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTMB340_ZFcharm_Kv1_Prnp_DPM

   Plasmid

   #220843

   Purpose

   AAV genome expressing ZFcharm Kv1 targeting Prnp; double perfect match self-silencing binding site

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm Kv1
   

   Use

   AAV

   Tags

   HAtag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

   Plasmid

   #71218

   Purpose

   dCas9 fused to the human DNMT3A catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3A(E752A)
   

   Use

   Lentiviral

   Tags

   SPdCas9-DNMT3A(E752A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-hSyn-SpdCas9-W3SL

   Plasmid

   #210708

   Purpose

   This Plasmid express hSyn promoter driven SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   hSyn

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. kanMX6-ins6

   Plasmid

   #195043

   Purpose

   pFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHage2-EF1a-mCherryTRF1-IRES-Blast

   Plasmid

   #182588

   Purpose

   expresses mcherry-trf1 in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hTRF1 (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. px330-CIB1-dCas9

   Plasmid

   #63665

   Purpose

   Mammalian gRNA expression vector also expressing CIB1-dCas9

   Depositor

   Dong Kong

   Article

   Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and CMV

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSUMO CPTP

    Plasmid

    #170735

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    CPTP (CPTP Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. natMX-ins7

    Plasmid

    #195044

    Purpose

    pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    NatR
    

    Use

    Yeast genomic targeting

    Tags

    tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. T7pr-His6-MBP-TEV-dCas9-BirA*

    Plasmid

    #159994

    Purpose

    Bacterial expression of dCas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    dCas9-BirA*
    

    Use

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. dCas9-NLS-gs10-cDHFR174

    Plasmid

    #188256

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cDHFR174 (DHFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV_SEPT9_i1 D1-25-msfGFP

    Plasmid

    #180333

    Purpose

    mammalian expression of human SEPT9_i1 D1-25 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 D1-25

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

    Plasmid

    #166809

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1322R

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid

    #68223

    Purpose

    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    35S

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTE4565

    Plasmid

    #88903

    Purpose

    Expresses inactive/dead, humanized MbCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(D986A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFLAG R106L-CPTP

    Plasmid

    #170744

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    CPTP (CPTP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R106L

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFLAG K60A-CPTP

    Plasmid

    #170743

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

    Insert

    CPTP (CPTP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K60A

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Islr-AP-His

    Plasmid

    #71950

    Purpose

    Expresses the entire Islr protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Islr (Islr Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge