We narrowed to 22,861 results for: sequence

1. pETM-11-DmPABPC1_501-634_J

   Plasmid

   #146641

   Purpose

   Bacterial Expression of HsPABPC1_501-634

   Depositor

   Elisa Izaurralde

   Insert

   HsPABPC1_501-634
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   2 silent mutations compared to the sequence given…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-HsTNRC6C_G

   Plasmid

   #146374

   Purpose

   Insect Expression of HsTRC6C

   Depositor

   Elisa Izaurralde

   Insert

   HsTRC6C
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two silent mutations C2349T and A2547C compared t…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-Opa1

   Plasmid

   #191961

   Purpose

   Gateway Destination Vector, containing OPA1 coding sequence, for Gateway Cloning Technology

   Depositor

   Luca Scorrano

   Insert

   Opa1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOME0276M

   Plasmid

   #182614

   Purpose

   Reversed sequence of Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. TO_7 Control for pOME0275

   Depositor

   Dirk Dittmer

   Insert

   Reverse compliment HHV8 TO_7 control
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOME0237M

   Plasmid

   #182599

   Purpose

   Reverse complement sequence of anti-PAN pOME0236 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.

   Depositor

   Dirk Dittmer

   Insert

   Reverse compliment of anti-PAN (pOME0236)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCGMbX

   Plasmid

   #42300

   Depositor

   Steven Almo

   Type

   Empty backbone

   Use

   Tags

   BiP signal sequence, His6, and V5-epitope

   Expression

   Insect

   Mutation

   Promoter

   metallothonein

   Available since

   April 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORFE0011

   Plasmid

   #112075

   Purpose

   pcUBI promoter module Pro+5U

   Depositor

   Christian Chevalier

   Insert

   pcUBI promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI and BpiI restriction sites have been remove…

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPINH

   Plasmid

   #53537

   Purpose

   pTriex-based vector with signal sequence and N-his tag for protein expression

   Depositor

   Ray Owens

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-flox-R4R3

   Plasmid

   #139517

   Purpose

   Provides two loxP sequences between gene cassettes in the destination vector

   Depositor

   Minoru Ko

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJPN0017

    Plasmid

    #112082

    Purpose

    eCFP module CDS1ns

    Depositor

    Christian Chevalier

    Insert

    eCFP without stop codon
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BpiI restriction sites have been remove…

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL2- Empty control -SV40-Luc

    Plasmid

    #26280

    Purpose

    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFX-β-tubulin-mCherry

    Plasmid

    #175829

    Purpose

    Express mCherry with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with microtuble-targeting sequence
    

    Use

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-Ubiquitin

    Plasmid

    #18712

    Purpose

    Mammalian expression of human ubiquitin with HA tag

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

    Insert

    ubiquitin (UBB Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The HA-Ubiquitin insert is from the UBB gene. The…

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. anti-FLAG M2 heavy chain

    Plasmid

    #175359

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 heavy chain
    

    Use

    Tags

    His8 tag and N-terminal secretion signal peptide …

    Expression

    Mammalian

    Mutation

    L51I (see paper)

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

    Plasmid

    #196415

    Purpose

    Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smMyc
    

    Use

    AAV; Astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and GfaABC1D

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_NEUROD1_P2A_Hygro_Barcode

    Plasmid

    #120466

    Purpose

    Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    NEUROD1 (NEUROD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PrimeRoot.v2C-Cre

    Plasmid

    #201739

    Purpose

    For DNA insertion test in protoplast

    Depositor

    Caixia Gao

    Article

    Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    ePPE,Cre
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-ERK1

    Plasmid

    #14747

    Depositor

    Rony Seger

    Article

    Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

    Insert

    ERK1 (MAPK3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. Factor IX donor

    Plasmid

    #182141

    Purpose

    Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-hAR-L26A,F27A

    Plasmid

    #89084

    Purpose

    mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    hAR FxxLF mutant (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR-L26A,F27A

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only