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pTrc99S-ssDsbA-TTc-4xDQNAT

Plasmid

#128401

Purpose

Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin fragment C domain

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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ptAD-seq-ubi63E-Gal4-DBD

Plasmid

#111930

Purpose

Vector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.

Depositor

Alexander Stark

Article

Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

Insert

tAD-seq Gal4-DBD

Expression

Insect

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFlp-Gm

Plasmid

#222335

Purpose

Expresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sacB (sacB B. subtilis)
flp (flp Budding Yeast)
cI (cI )

Promoter

cl, flp, and sacB

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG

Plasmid

#127335

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Axin2-d2EGFP

Plasmid

#21276

Depositor

Frank Costantini

Article

Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Insert

Axin2 promoter (Axin2 Mouse)

Tags

d2EGFP

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pFlp-Km

Plasmid

#222334

Purpose

Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sacB (sacB B. subtilis)
flp (flp Budding Yeast)
cI (cI )

Promoter

cl, flp, and sacB

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-NF186WT-HA

Plasmid

#239774

Purpose

Expresses WT rat NF186 with a C-terminal HA tag in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAdTrack-CMV FLAG Rev-Erb alpha

Plasmid

#22745

Depositor

Bruce Spiegelman

Article

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

Rev Erb alpha (NR1D1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pDL025_HA-rb_IgG_HC_15F11

Plasmid

#193622

Purpose

HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti_HA_15F11 rabbit heavy chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDL026_HA-rb_IgG_LC_15F11

Plasmid

#193623

Purpose

HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-HA_15F11 rabbit light chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-CHIKV-nsp3-c3xFLAG

Plasmid

#215696

Purpose

Produces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp3

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBV65

Plasmid

#108363

Purpose

A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

Depositor

Scott Moye-Rowley

Article

Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

Insert

loxP-natMX6-CgMET3prom-cre-loxP

Use

Gene disruption marker for candida glabrata

Promoter

C. glabrata MET3

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

Plasmid

#156440

Purpose

For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA1 (Stag1 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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145-pGL3ctrl-3' UTR

Plasmid

#28175

Depositor

Michael Kastan

Article

Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

Insert

Luciferase flanked by tp53 UTRs (TP53 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Note that insert length is based on sum of the 2 …

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Frankenbody-HA-mScarlet3-IRESv4-HaloTag-tdMCP

Plasmid

#241135

Purpose

Expresses Anti-HA frankenbody-mScarlet3 and HaloTag-tdMCP to track mature and nascent HA-tagged proteins and MS2-tagged mRNA

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-HA frankenbody
tdMCP

Tags

HaloTag and mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pICSL11059

Plasmid

#68263

Purpose

Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

Plasmid

#127332

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Sstr3-GFP-DEST

Plasmid

#49098

Purpose

Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Sstr3 (Sstr3 Mouse)

Tags

GFP and acceptor peptide (AP)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

Plasmid

#52521

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

AAV and Cre/Lox

Tags

2xHA on ChR2

Promoter

CBA

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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