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Showing: 3921 - 3940 of 6460 results

  3. mCerulean-proα2(I)-G610C

    Plasmid
    #119837
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119837-plasmid-map-sequence-id-233867

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  9. pcDNA3.BRWD3-V5H

    Plasmid
    #48624
    Purpose
    Expresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cells
    Depositor
    Aziz Sancar
    Article
    Ozturk et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    BRWD3 (BRWD3 Fly)
    Tags
    V5 and His tags
    Expression
    Mammalian
    Mutation
    no mutations, stop was removed to fuse V5His at C…
    Promoter
    CMV
    Available Since
    Oct. 15, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. A24M04 mIgM heavy chain-pFEEKW

    Plasmid
    #234809
    Purpose
    Express membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1
    Depositor
    Susan Pierce
    Article
    Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12
    Insert
    A24M04 membrane IgM heavy chain (IGHM Human)
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Promoter
    EEK promoter
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    234809-plasmid-map-sequence-id-466813

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  14. pDY118A

    Plasmid
    #182958
    Purpose
    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Inserts
    Cas9
    Gam, Beta, Exo
    I-scel
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182958-plasmid-map-sequence-id-365360

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  17. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid
    #220611
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220611-plasmid-map-sequence-id-441218

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  19. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162682-plasmid-map-sequence-id-320836

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Showing: 3921 - 3940 of 6460 results