We narrowed to 24,463 results for: pET

1. pET/ELP-I-CAT

   Plasmid

   #71461

   Purpose

   ELP-intein purification system

   Depositor

   David Wood

   Article

   Banki et al Nat Methods. 2005 Sep;2(9):659-61.

   Type

   Empty backbone

   Use

   Tags

   ELP ((VPGXG)110) and intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b His6-FKBPF36V

   Plasmid

   #73180

   Purpose

   Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

   Depositor

   Thomas Wandless

   Article

   Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

   Insert

   FKBP(F36V) (FKBP1A Human)
   

   Use

   Tags

   His 6x

   Expression

   Bacterial

   Mutation

   F36V

   Promoter

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pET16b-His-ArgRS

   Plasmid

   #124104

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Argin+I4 ligase (argS Escherichia coli)
   

   Use

   Tags

   10x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a-MetRS-His

   Plasmid

   #124115

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Methionine--tRNA ligase (metG Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-HaloTag7-SnoopLigase

   Plasmid

   #105627

   Purpose

   Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7

   Depositor

   Mark Howarth

   Article

   Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

   Insert

   HaloTag7-SnoopLigase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-His-IleRS

   Plasmid

   #124112

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Isoleucyl-tRNA ligase (ileS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-LeuRS-His

   Plasmid

   #124113

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Leucyl-tRNA ligase (leuS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-TrpRS-His

   Plasmid

   #124120

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Tryptophanyl-tRNA ligase (trpS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21a-GlyRS-His

   Plasmid

   #124110

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a-HisRS-His

    Plasmid

    #124111

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Histidyl-tRNA ligase (hisS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a-CysRS-His

    Plasmid

    #124107

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Cysteinyl-tRNA ligase (cysS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET24a-AlkB deltaN11

    Plasmid

    #73622

    Purpose

    Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

    Depositor

    Catherine Drennan

    Article

    Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    AlkB (alkB E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deletion of first 11 AA

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pET21a-MTF-His

    Plasmid

    #124133

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionyl-tRNA formyltransferase (fmt Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET259-pUC57 24xMS2V6

    Plasmid

    #104391

    Purpose

    MS2V6 cloning

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b PKA Cat

    Plasmid

    #14921

    Depositor

    Susan Taylor

    Article

    Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

    Insert

    PKA catalytic subunit alpha (Prkaca Mouse)
    

    Use

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET30a-His6-LOXCAT

    Plasmid

    #140084

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a HAT epitope

    Plasmid

    #129445

    Purpose

    expression of HAT epitope

    Depositor

    Oskar Laur

    Insert

    HAT epitope
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-32a(+)-NRG1B1

    Plasmid

    #120287

    Purpose

    Produce human recombinant NRG1 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    NRG1-beta 1
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29b-AK1-His

    Plasmid

    #124135

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Adenylate kinase (AK1 Chicken)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-SBP-TEV

    Plasmid

    #36943

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHistag-SBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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