Skip to main content
Addgene
Showing: 5941 - 5970 of 8427 results

  1. pCfB8379

    Plasmid
    #126913
    Purpose
    Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB
    Depositor
    Irina Borodina
    Article
    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062
    Insert
    XdCrtI and XdCrtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    126913-plasmid-map-sequence-id-248795

    Enlarge

  4. LV TRE-GFP-TMD-DLL1

    Plasmid
    #194936
    Purpose
    LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.
    Depositor
    John Ngo
    Article
    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Insert
    eGFP-(PDGFR)TMD-rDLL1 ICD (WT)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194936-plasmid-map-sequence-id-386565

    Enlarge

  6. pAG25-L-IFP-F[1] (clon 3)

    Plasmid
    #52899
    Purpose
    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    L-IFP-F[1]
    Use
    Vector for homologous recombination
    Tags
    Expression
    Mutation
    Promoter
    TEF promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pAG32-L-IFP-F[2] (clon 3)

    Plasmid
    #52900
    Purpose
    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Article
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Insert
    L-IFP-F[2]
    Use
    Vector for homologous recombination
    Tags
    Expression
    Mutation
    Promoter
    TEF promoter
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pGL3-LRR

    Plasmid
    #66937
    Purpose
    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Reverse orientation (attR2, attR1).
    Depositor
    Alvaro Monteiro
    Article
    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV-40
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pARNTL-donor

    Plasmid
    #112384
    Purpose
    CRISPR donor plasmid to tag human transcription factor ARNTL with GFP
    Depositor
    Kevin White
    Insert
    ARNTL homology arms flanking EGFP-IRES-Neo cassette (BMAL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112384-plasmid-map-sequence-id-229842

    Enlarge

  12. pTFAP2C-donor

    Plasmid
    #113824
    Purpose
    CRISPR donor plasmid to tag human transcription factor TFAP2C with GFP
    Depositor
    Kevin White
    Insert
    TFAP2C homology arms flanking EGFP-IRES-Neo cassette (TFAP2C Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs6025024, hg38:20:56638660:TTT/-
    Promoter
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113824-plasmid-map-sequence-id-230196

    Enlarge

  15. pMAZ-donor

    Plasmid
    #112359
    Purpose
    CRISPR donor plasmid to tag human transcription factor MAZ with GFP
    Depositor
    Kevin White
    Insert
    MAZ homology arms flanking EGFP-IRES-Neo cassette (MAZ Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:16:29809765:G>A
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112359-plasmid-map-sequence-id-229679

    Enlarge

  16. ABCB8 (ABCB8A-c016)

    Plasmid
    #110266
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ABCB8 (ABCB8 Human)
    Use
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110266-plasmid-map-sequence-id-212276

    Enlarge

  22. GGE038

    Plasmid
    #120785
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsDown_Zeta-NotI
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120785-plasmid-map-sequence-id-263183

    Enlarge

  23. GGE067

    Plasmid
    #120784
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly
    Depositor
    Tristan Rossignol
    Article
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    InsUp_Zeta-NotI
    Use
    Cloning/assembly plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120784-plasmid-map-sequence-id-263182

    Enlarge

  25. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid
    #69548
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69548-plasmid-map-sequence-id-122974

    Enlarge

  27. pICSL70002

    Plasmid
    #50333
    Depositor
    Nicola Patron
    Article
    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43
    Insert
    Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
    Use
    Plant expression
    Tags
    Expression
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Promoter
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    50333-plasmid-map-sequence-id-194302

    Enlarge

Showing: 5941 - 5970 of 8427 results