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pAc5.1B-EGFP-Dm4E-T-del28-43_T

Plasmid

#147601

Purpose

Insect Expression of Dm4E-T-del28-43

Depositor

Elisa Izaurralde

Publication

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-del28-43 (4E-T Fly)

Expression

Insect

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T-del28-43_T

Plasmid

#147609

Purpose

Insect Expression of Dm4E-T-del28-43

Depositor

Elisa Izaurralde

Publication

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-del28-43 (4E-T Fly)

Expression

Insect

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T_1-58_T

Plasmid

#147595

Purpose

Insect Expression of Dm4E-T_1-58

Depositor

Elisa Izaurralde

Publication

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-58 (4E-T Fly)

Expression

Insect

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1963-2505-dsRNAres_AC

Plasmid

#148403

Purpose

Insect Expression of Dmot1_1963-2502-dsRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

Dmot1_1963-2502-dsRNAres

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmRRP6_AA

Plasmid

#148220

Purpose

Insect Expression of DmRRP6

Depositor

Elisa Izaurralde

Insert

DmRRP6 (Rrp6 Fly)

Expression

Insect

Mutation

two non silent muations S645 to P and A752 to V c…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-148-464

Plasmid

#136021

Purpose

EIF3B (148-464) 3'UTR fragment inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Publication

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-465-552

Plasmid

#136022

Purpose

EIF3B (465-552) 3'UTR fragment inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Publication

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-148-552

Plasmid

#136024

Purpose

EIF3B (148-552) 3'UTR fragment inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Publication

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUG-125

Plasmid

#104636

Purpose

Level 2 construct

Depositor

Marco Trujillo

Publication

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB-F/A_EL4

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Mutation

AvrPtoB-Phe479Ala

Promoter

T7

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-412

Plasmid

#104638

Purpose

Level 2 construct

Depositor

Marco Trujillo

Publication

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_Myc-Fen_EL5

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Promoter

T7

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Ccdc167

Plasmid

#120242

Purpose

Expresses V5-tagged Ccdc167 in lentiviral vector

Depositor

Larry Gerace

Insert

Ccdc167 (Ccdc167 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0004

Plasmid

#117579

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0023)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0003

Plasmid

#117578

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_NbPDS(pEPOR1CB0022)

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mIFITM3-Y20,27A

Plasmid

#58465

Purpose

Expresses murine IFITM3-Y20,27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Publication

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosines 20 and 27 to alanine

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3-Y27A

Plasmid

#58464

Purpose

Expresses murine IFITM3-Y27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Publication

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 27 to alanine

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
p198 Dync1i2.F (Ex1a)

Plasmid

#26448

Depositor

Elizabeth Fisher

Publication

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform F (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
sg_Snrnp48_i1-ipUSEPR-TR657

Plasmid

#228925

Purpose

Knockdown of Snrnp48 in mammalian cells

Depositor

Sika Zheng

Publication

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Snrnp48_i1 (Snrnp48 Mouse)

Use

Lentiviral

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WTSLFD

Plasmid

#231881

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WTSLFD

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WTSLFD

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108W, E109T, Y110S, E111L, N112F, L113D

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Tada3_i3-ipUSEPR-TR657

Plasmid

#228915

Purpose

Knockdown of Tada3 in mammalian cells

Depositor

Sika Zheng

Publication

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Tada3_i3 (Tada3 Mouse)

Use

Lentiviral

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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sg_Tada3_i5-ipUSEPR-TR657

Plasmid

#228916

Purpose

Knockdown of Tada3 in mammalian cells

Depositor

Sika Zheng

Publication

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Tada3_i3 (Tada3 Mouse)

Use

Lentiviral

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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