We narrowed to 34,977 results for: CaS

1. pENTR-L3-Csy4-FokI-dCas9-EGFP-L2

   Plasmid

   #141047

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Csy4-2A-FokI-dCas9-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLS

   Plasmid

   #121827

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-Cas9 (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. A3H HapII R175/6E-Cas9n-UGI

   Plasmid

   #119142

   Purpose

   Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   A3H Hap II RR175/6EE

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MEI-S332-GFP genomic in pCaSpeR

   Plasmid

   #113174

   Purpose

   In P element vector for transposon insertion into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

   Insert

   mei-S332 (Sgo)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459V2.0-eSpCas9(1.1)+K810A+K855A

   Plasmid

   #108299

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCASCADE-gltA1-gltA2-udhA-zwf

   Plasmid

   #87152

   Purpose

   gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   gltA1-gltA2-udhA-zwf guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCASCADE-gltA1-gltA2-udhA-GapAP1

   Plasmid

   #87151

   Purpose

   gltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   gltA1-gltA2-udhA-GapAP1 guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCASCADE-fabI-gltA1-gltA2-gapAP1

   Plasmid

   #87147

   Purpose

   fabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   fabI-gltA1-gltA2-gapAP1 guide array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-dCas9-p300 Core (C1204R)

   Plasmid

   #61361

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; C1204R mutation i…

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAD05 S. castellii telomerase RNA KBS

    Plasmid

    #61894

    Purpose

    Plasmid for run-off in vitro transcription of Saccharomyces castellii KBS RNA

    Depositor

    Thomas Cech

    Article

    Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

    Insert

    Telomerase RNA hairpin
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. RCAS (B) flag Radical Fringe (CT#472)

    Plasmid

    #13983

    Depositor

    Cliff Tabin

    Article

    Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

    Insert

    Radical Fringe (RFNG Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

12. CROPseq-Guide-EFS-SpCas9-P2A-EGFP

    Plasmid

    #99248

    Purpose

    single-vector system with EGFP reporter for use in CROPseq knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3TS-spCas9 ABE-Umax-ex2-rest1

    Plasmid

    #222143

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3TS-spCas9 ABE-Umax-ex1-rest1

    Plasmid

    #222140

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-Cas9-T2A-EGFP-ires-puro

    Plasmid

    #78311

    Purpose

    Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

    Depositor

    Timo Otonkoski

    Article

    Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

    Insert

    WT SpCas9-T2A-EGFP-ires-puromycin resistance
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

    Plasmid

    #108871

    Purpose

    Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Inserts

    5xU6:sgRNA
    Cas9-t2A-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and hsp70

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid

    #118156

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid

    #171588

    Purpose

    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

    Plasmid

    #64216

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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