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pGMCtKq28-TSC10M1-sspB

Plasmid

#49526

Purpose

Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

TSC10M1-sspB

Expression

Bacterial

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pMMS22L-siR3

Plasmid

#221013

Purpose

mammalian expression vector of myc-Flag tagged MMS22L siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (MMS22L Human)

Tags

Flag and myc

Expression

Mammalian

Mutation

silent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…

Promoter

CMV

Available Since

July 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDNA5-FRT/TO-FLAG-TONSL

Plasmid

#221011

Purpose

mammalian expression vector of Flag tagged TONSL siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

TONSL (TONSL Human)

Tags

Flag

Expression

Mammalian

Mutation

silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

Promoter

CMV

Available Since

June 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Venus-rab11a

Plasmid

#234744

Purpose

Mammalian expression of recycling endosome marker Venus-rab11a

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-rab11a (RAB11A Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-qmTQ2-ATP

Plasmid

#225248

Purpose

mammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP3654

Plasmid

#222349

Purpose

Expression vector with GFP expressed under PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pKH116_keima-YIPF3

Plasmid

#214970

Purpose

Golgiphagy flux reporter keima-YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

keima-YIPF3 (YIPF3 Human)

Use

Lentiviral

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKH118_keima-YIPF4

Plasmid

#214971

Purpose

Golgiphagy flux reporter keima-YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

keima-YIPF4 (YIPF4 Human)

Use

Lentiviral

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA63-GG

Plasmid

#208015

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 puro

Plasmid

#206215

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX303 GFP-PIK3C2B

Plasmid

#162001

Purpose

Lentiviral expression vector containing PIK3C2B tagged with GFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2B (PIK3C2B Human)

Use

Lentiviral

Tags

eGFP

Mutation

siRNA resistance to siRNA#2 with 4 silent mutatio…

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pTalpha1-iCre

Plasmid

#133924

Purpose

Neuron-specific expression of iCre

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

iCre

Expression

Mammalian

Mutation

Codon optimized

Promoter

neuron-specific tubulin-alpha-1 promoter

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-ICP0FxE-C3

Plasmid

#134561

Purpose

Expresses YFP-ICP0 fusion protein with a RING finger deletion (FXE)

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

ICP0

Tags

YFP

Expression

Mammalian

Mutation

RING finger deletion (FXE)

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shID2 #2

Plasmid

#83087

Purpose

Lentiviral shRNA vector for inducible knockdown of human ID2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shID2 (ID2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shSMAD4 #2

Plasmid

#83091

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pUC19-U6-AasgRNA

Plasmid

#121954

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔPRYSPRY

Plasmid

#105521

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Mutation

deletion of PRYSPRY domain (aa1-281)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pJC-repo1.9kb-TALE4-VP64-P10

Plasmid

#104612

Purpose

Expresses TALE4 under the control of a 1.9kb repo enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

1.9kb repo enhancer element (repo Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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NsvBa

Plasmid

#100015

Purpose

Electrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFP

Depositor

David Clapham, Paul DeCaen

Article

DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

Insert

NsvBa

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGLACTE-HT51-13

Plasmid

#99256

Purpose

Contains normal CGG repeats (29 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. To generate capillary electrophoresis size standards.

Depositor

Karen Usdin

Article

Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

Insert

FMR1 (FMR1 Human)

Tags

eGFP

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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