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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,172 results for: PEP

Showing: 3961 - 3980 of 5172 results

  1. pYUBcLIC-GFP

    Plasmid
    #183509
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    183509-plasmid-map-sequence-id-362497

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  5. pSCM167.2-Venus-VF-hADAM10(aa1-654)

    Plasmid
    #189558
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only prodomain through cysteine rich domain--amino acids 1-654
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa1-654
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189558-plasmid-map-sequence-id-374390

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  7. pTS2393-Tier1-PhCMV-p2a_fastFT

    Plasmid
    #169530
    Purpose
    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven P2A-fused fluorescent timer, fast maturation time
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169530-plasmid-map-sequence-id-366213

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  8. pTS2392-Tier1-PhCMV-p2a_medFT

    Plasmid
    #169529
    Purpose
    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven P2A-fused fluorescent timer, medium maturation time
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169529-plasmid-map-sequence-id-364611

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  10. pTS2387-Tier1-PhCMV-p2a_mTagBFP2

    Plasmid
    #169524
    Purpose
    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169524-plasmid-map-sequence-id-364607

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  14. pDONR207-MUM2sp-PelA

    Plasmid
    #170722
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    Pectin lyase A
    Use
    Gateway donor vector / entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 20, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170722-plasmid-map-sequence-id-345737

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  15. pDONR207-MUM2sp-RglB

    Plasmid
    #170721
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    Rhamnogalacturonan lyase B
    Use
    Gateway donor vector / entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 20, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170721-plasmid-map-sequence-id-345736

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  16. pDONR207-MUM2sp-RglA

    Plasmid
    #170720
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    Rhamnogalacturonan lyase A
    Use
    Gateway donor vector / entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 20, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170720-plasmid-map-sequence-id-345734

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  17. pLyGo-Bs-4

    Plasmid
    #163142
    Purpose
    pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PamyL-PamyQ-PcryIIIA-cryIIIAstab49
    Available since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    163142-plasmid-map-sequence-id-338663

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  18. pLyGo-Ec-4

    Plasmid
    #163130
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    163130-plasmid-map-sequence-id-338661

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Showing: 3961 - 3980 of 5172 results