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pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196083

Purpose

Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196075

Purpose

Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196077

Purpose

Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_346

Plasmid

#180549

Purpose

Entry vector containing gfasPurple

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

gfasPurple

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_316

Plasmid

#180547

Purpose

Entry vector containing sfGFP

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sfGFP

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_344

Plasmid

#180548

Purpose

Entry vector containing cjBlue

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

cjBlue

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_345

Plasmid

#180550

Purpose

Entry vector containing eforRed

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

eforRed

Expression

Bacterial

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_036

Plasmid

#180558

Purpose

Entry vector containing L3S1P13 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P13 terminator

Expression

Bacterial

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_009

Plasmid

#180518

Purpose

Empty backbone for D1.1 cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_137

Plasmid

#180521

Purpose

Empty backbone for D1.2 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_010

Plasmid

#180519

Purpose

Empty backbone for D1.2 cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_040

Plasmid

#180562

Purpose

Entry vector containing L3S2P24 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P24 terminator

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_048

Plasmid

#180564

Purpose

Entry vector containing L3S1P00

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P00_E1.4b

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_029

Plasmid

#180542

Purpose

Entry vector containing RBS BBa_B0035

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

RBS BBa_B0035

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_035

Plasmid

#180557

Purpose

Entry vector containing L3S1P00 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P00 terminator

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_033

Plasmid

#180545

Purpose

Entry vector containing LuxR

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

LuxR

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_037

Plasmid

#180559

Purpose

Entry vector containing L3S1P22 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P22 terminator

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_003

Plasmid

#180541

Purpose

Entry vector containing RBS BBa_B0034

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

RBS BBa_B0034

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_016

Plasmid

#180523

Purpose

Empty backbone for D2.2b cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_015

Plasmid

#180522

Purpose

Empty backbone for D2.2a cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge