We narrowed to 3,100 results for: AER

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pET28b-pvdA WT

Plasmid

#87098

Purpose

expresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Olucha et al J Biol Chem. 2011 Sep 9;286(36):31789-98

Insert

pvdA WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-pchA WT

Plasmid

#87102

Purpose

expresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Arch Biochem Biophys. 2013 Oct 1;538(1):

Insert

pchA WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pHisIHFαIHFβ

Plasmid

#149384

Purpose

Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Inserts

Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta

Tags

6xHis tag and HRV3C cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMA3200

Plasmid

#46855

Purpose

A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY and myc-tagged destabilized FKBP12

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

ExoY

Use

Lentiviral

Tags

FKBP E31G, R71G, K105E and Myc

Promoter

TRE-Tight

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-FRB

Plasmid

#194455

Purpose

Mammalian expression vector that expresses the HIV-1 GAG protein with FRB fused to it

Depositor

Sven Eyckerman

Article

Bogaert et al Methods Enzymol. 2023;684:253-287. doi:

Insert

FRB (MTOR Human)

Tags

GAG

Expression

Mammalian

Promoter

SR alpha

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-pchD WT

Plasmid

#187973

Purpose

Expresses salicylate adenylation enzyme PchD from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Shelton et al J Biol Inorg Chem. 2022 May 5. pii: 10.1

Insert

pchD WT

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_wt

Plasmid

#149386

Purpose

P. aeruginosa PA14 CRISPR2 locus

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMA3228

Plasmid

#46856

Purpose

A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

ExoY K81M

Use

Lentiviral

Tags

FKBP E31G, R71G, K105E and Myc

Promoter

TRE-Tight

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pSG058

Plasmid

#214261

Purpose

PaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+1D

Plasmid

#149389

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

1bp inserted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pSG069

Plasmid

#214260

Purpose

pJN105 with PaftsH2SD and PaFtsH2 ORF cloned between NheI/XbaI

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

tLST accessory genetic element encoded PaFtsH2 proteinase from Pseudomonas aeruginosa SG17M

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IRdist_scram

Plasmid

#162319

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

The distal Upstream Motif site is scrambled

Promoter

pBAD

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_-6D

Plasmid

#149387

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

6bp deleted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_-5D

Plasmid

#149388

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

5bp deleted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+5D

Plasmid

#149390

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

5bp inserted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+10D

Plasmid

#149391

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

10bp inserted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHF_Opt

Plasmid

#149395

Purpose

P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

IHFprox site is optimized at key positions needed…

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_motif_scram

Plasmid

#149397

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

Upstream motif recognized by Cas1-2/3 during spac…

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMET7-EGFP-FKBP12

Plasmid

#194453

Purpose

Mammalian expression vector that expresses the EGFP protein with FKBP12 fused to it

Depositor

Sven Eyckerman

Article

Bogaert et al Methods Enzymol. 2023;684:253-287. doi:

Insert

FKBP12 (FKBP1A Human)

Tags

eGFP

Expression

Mammalian

Promoter

SR alpha

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMET7-EDHFR-FKBP12

Plasmid

#194454

Purpose

Mammalian expression vector that expresses the EDHFR protein with FKBP12 fused to it

Depositor

Sven Eyckerman

Article

Bogaert et al Methods Enzymol. 2023;684:253-287. doi:

Insert

FKBP12 (FKBP1A Human)

Tags

eDHFR

Expression

Mammalian

Promoter

SR alpha

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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