We narrowed to 20,240 results for: ENA;

1. pCMV enAsCas12f-HKRA-sgRNA_v7

   Plasmid

   #208357

   Purpose

   Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-HKRA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I123H/D195K/D208R/V232A

   Promoter

   CMV

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV enAsCas12f-YHAM-sgRNA_v7

   Plasmid

   #208356

   Purpose

   Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-YHAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F48Y/S188H/V232A/E316M

   Promoter

   CMV

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCH4 (denAsCas12a-KRAB lenti)

   Plasmid

   #217331

   Purpose

   Lentiviral expression of denAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   denAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mutation

   D908A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCH62 (denAsCas12a-KRAB piggyBac)

   Plasmid

   #217333

   Purpose

   piggyBac expression of denAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   denAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   D908A/E174R/S542R/K548R

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENAAV-cTNT-actn2-BioID2-HA

   Plasmid

   #216831

   Purpose

   cardiomyocytes-specific plasmid, expressing the coding sequence of mouse Actn2, biotin ligase BioID2, and a HA tag.

   Depositor

   Yuxuan Guo

   Article

   Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

   Insert

   BioID-HA (Actn2 Synthetic)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA

   Plasmid

   #68346

   Purpose

   Used to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

   Inserts

   3xgRNA;PTENA, p53B,SMAD4A
   FlPO
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNS49 (enAsCas12a-KRAB piggyBac)

   Plasmid

   #217335

   Purpose

   piggyBac expresion of enAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   enAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   E174R/S542R/K548R

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

   Plasmid

   #114084

   Purpose

   CAG promoter expression plasmid for human codon optimized enAsBE1.4

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
   

   Use

   Tags

   2x SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A, human codon optimi…

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

   Plasmid

   #114081

   Purpose

   CAG promoter expression plasmid for human codon optimized enAsBE1.1

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A, human codon optimi…

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET47b-T7-enAsCas12f

    Plasmid

    #204642

    Purpose

    Expression of enAsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    enAsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D196K, N199K, G276R, N328G, D364R

    Promoter

    T7

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-enAsCas12f

    Plasmid

    #204637

    Purpose

    Expression of enAsCas12f in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    enAsCas12f
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D196K, N199K, G276R, N328G, D364R

    Promoter

    CMV

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. h-alpha-ENaC

    Plasmid

    #83430

    Purpose

    full length human alpha ENaC with Flag tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2008 May;294

    Insert

    human alpha ENaC (SCNN1A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. h-beta-ENaC

    Plasmid

    #83429

    Purpose

    full length human beta ENaC with V5 tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2008 May;294

    Insert

    human beta ENaC (SCNN1B Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. enAsCas12a-mSA

    Plasmid

    #182954

    Purpose

    Express enAsCas12a-mSA

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    enAsCas12a-mSA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. h-alpha-ENaC-2

    Plasmid

    #83431

    Purpose

    full length human alpha ENaC-2 with flag tag

    Depositor

    Christie Thomas

    Article

    Thomas et al Am J Physiol. 1998 May;274(5 Pt 1):C1312

    Insert

    human alpha ENaC isoform 2 (SCNN1A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. enAsABE8e-dimer

    Plasmid

    #138507

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-dAsCas12a (En)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid

    #114082

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    

    Use

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. penaav-tnt-3xha-p2a-gfp

    Plasmid

    #175090

    Purpose

    For making AAV9

    Depositor

    William Pu

    Article

    Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3xNLS-NLP-cMyc-cMyc enAspCas12a

    Plasmid

    #182127

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc enAspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

    Plasmid

    #182126

    Purpose

    pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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